PCGF1在直肠腺癌中高表达，沉默PCGF1可抑制直肠腺癌细胞的增殖。
[PCGF1 is highly expressed in rectal adenocarcinoma and silencing PCGF1 inhibits proliferation of rectal adenocarcinoma cells ].
作者信息
Zeng Y, Fan R
机构信息
Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Central Laboratory, Tianjin Xiqing Hospital, Tianjin 300380, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2022 Sep 20;42(9):1296-1302. doi: 10.12122/j.issn.1673-4254.2022.09.04.
OBJECTIVE
To investigate the expression of PCGF1 in rectal adenocarcinoma (READ) and the effect of PCGF1 silencing on proliferation READ cells in vitro.
METHODS
The UALCAN and ENCORI online databases were used to analyze the expression level of PCGF1 in READ tissues and normal tissues and its association with the clinicopathological parameters and survival outcomes of patients with READ. The expression levels of PCGF1 were detected in two READ cell lines and a normal rectal epithelial cell line (HcoEpiC cells) using qPCR and Western blotting. Lentiviral vectors were used to construct PCGF1-overexpressing and PCGF1-silenced cell lines, and the proliferative activity of the cells was assessed using CCK-8 assay. The effect of PCGF1 silencing on tumor proliferation was also evaluated by observing tumorigenicity of the cells in nude mice.
RESULTS
PCGF1 was highly expressed in READ tissue ( < 0.001), and its expression levels was correlated with READ stage, differentiation and lymph node metastasis ( < 0.001). A high PCGF1 expression level was associated with a poor survival outcome of READ patients ( < 0.05). In SW837 and SW1463 cells, PCGF1 silencing significantly lowered the proliferative activity of the cells both ( < 0.05) and in nude mice ( < 0.01).
CONCLUSION
PCGF1 is highly expressed in READ tissue and may potentially serve as a prognostic biomarker as well as a therapeutic target for READ.
目的
研究PCGF1在直肠腺癌（READ）中的表达以及PCGF1沉默对体外READ细胞增殖的影响。
方法
使用UALCAN和ENCORI在线数据库分析PCGF1在READ组织和正常组织中的表达水平及其与READ患者临床病理参数和生存结果的关联。使用qPCR和蛋白质印迹法检测两种READ细胞系和一种正常直肠上皮细胞系（HcoEpiC细胞）中PCGF1的表达水平。使用慢病毒载体构建PCGF1过表达和PCGF1沉默的细胞系，并使用CCK-8法评估细胞的增殖活性。还通过观察细胞在裸鼠中的致瘤性来评估PCGF1沉默对肿瘤增殖的影响。
结果
PCGF1在READ组织中高表达（<0.001），其表达水平与READ分期、分化程度和淋巴结转移相关（<0.001）。PCGF1高表达与READ患者的不良生存结果相关（<0.05）。在SW837和SW1463细胞中，PCGF1沉默显著降低了细胞在体外（<0.05）和裸鼠体内（<0.01）的增殖活性。
结论
PCGF1在READ组织中高表达，可能作为READ的预后生物标志物和治疗靶点。
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