Tang Bingqian, Li Xiumin, Zhang Xinhao, Yin Qinbiao, Xie LingLing, Zou Xuexiao, Liu Feng, Dai Xiongze
College of Horticulture, Hunan Agricultural University, Changsha, China.
Longping Branch, Graduate School of Hunan University, Changsha, China.
Front Plant Sci. 2022 Sep 21;13:946475. doi: 10.3389/fpls.2022.946475. eCollection 2022.
Climate change and global warming pose a great threat to plant growth and development as well as crop productivity. To better study the genome-wide gene expression under heat, we performed a time-course (0.5 to 24 h) transcriptome analysis in the leaf and root of 40-day-old pepper plants under 40°C as well as in control plants. Clustering analysis (K-means) showed that the expression of 29,249 genes can be grouped into 12 clusters with distinct expression dynamics under stress. Gene ontology (GO) enrichment analysis and transcription factor (TF) identification were performed on the clusters with certain expression patterns. Comparative analysis between the heat-treated and control plants also identified differentially expressed genes (DEGs), which showed the largest degree of change at 24 h. Interestingly, more DEGs were identified in the root than in the leaf. Moreover, we analyzed the gene expression of 25 heat shock factor genes (HSFs) in pepper after heat stress, identified five of these HSFs that responded to heat stress, and characterized the role of these genes in heat-tolerant (17CL30) and heat-susceptible (05S180) pepper lines. The findings of this study improve our understanding of the genome-wide heat stress response in pepper.
气候变化和全球变暖对植物的生长发育以及作物生产力构成了巨大威胁。为了更好地研究高温下全基因组的基因表达情况，我们对40日龄辣椒植株在40°C条件下以及对照植株的叶片和根部进行了时间进程（0.5至24小时）的转录组分析。聚类分析（K均值法）表明，29249个基因的表达可分为12个簇，在胁迫下具有不同的表达动态。对具有特定表达模式的簇进行了基因本体（GO）富集分析和转录因子（TF）鉴定。热处理植株与对照植株之间的比较分析还鉴定出了差异表达基因（DEG），这些基因在24小时时变化程度最大。有趣的是，根部鉴定出的DEG比叶片中更多。此外，我们分析了热胁迫后辣椒中25个热激因子基因（HSF）的基因表达，鉴定出其中5个HSF对热胁迫有响应，并对这些基因在耐热（17CL30）和热敏感（05S180）辣椒品系中的作用进行了表征。本研究结果增进了我们对辣椒全基因组热胁迫响应的理解。
