[关于……对细菌应激反应、细胞内感染及免疫的影响] （原文中“Effect of on”部分缺失具体内容）
[Effect of on Bacterial Stress Response and Intracellular Infection and Immunity].
作者信息
Wang Xin-Yan, Luo Tao, Chen Zong-Hai, Liao Wei, Wang Yi, Bao Lang
机构信息
Laboratory of Infection and Immunology, Department of Pathogenic Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2022 Sep;53(5):828-833. doi: 10.12182/20220960209.
OBJECTIVE
To investigate the effect of ( ) on bacterial stress response and intracellular infection and immunity.
METHODS
The target gene amplified from H37Rv genome was cloned to the vector and then transferred to ( ) to construct a recombinant strain. Stress response experiment and Raw264.7 mouse macrophage infection was carried out with , the recombinant strain, and , the vector strain. Tests were conducted to measure bacterial colony forming unit (CFU) and transcriptional levels of cytokines, including interleukin ( )-1β, -6, -10, -12 40, interferon ( )- , tumor necrosis factor ( )- , and inducible nitric oxide synthase ( ).
RESULTS
The recombinant strain, , was constructed successfully. According to the findings of the stress response experiment, could indeed enhance bacterial survival under certain conditions of culture. Intracellular infection experiment demonstrated that enhanced bacterial survival in macrophages and influenced the transcriptional level of cytokines.
CONCLUSION
The genes from play a role in bacterial stress response and intracellular infection and immunity.
目的
研究（ ）对细菌应激反应、细胞内感染及免疫的影响。
方法
从H37Rv基因组中扩增出目标基因，克隆至载体，然后转入（ ）构建重组菌株。用（ ）、重组菌株及载体菌株进行应激反应实验和Raw264.7小鼠巨噬细胞感染实验。检测细菌菌落形成单位（CFU）以及细胞因子的转录水平，包括白细胞介素（ ）-1β、-6、-10、-12 40、干扰素（ ）- 、肿瘤坏死因子（ ）- 以及诱导型一氧化氮合酶（ ）。
结果
成功构建了重组菌株（ ）。根据应激反应实验结果，（ ）确实能在特定培养条件下提高细菌存活率。细胞内感染实验表明，（ ）能提高细菌在巨噬细胞中的存活率，并影响细胞因子的转录水平。
结论
来自（ ）的（ ）基因在细菌应激反应、细胞内感染及免疫中发挥作用。
Zotero 插件