使用全长16S rRNA基因测序对儿童肺炎进行病因诊断的物种水平呼吸道微生物组分析

Species-level respiratory microbiome profiling for etiologic diagnosis of children pneumonia using full length 16S rRNA gene sequencing.

作者信息

Li Lifeng, Ma Jiayue, Li Min, Cheng Ping, Li Mingchao, Yu Zengyuan, Zhang Wancun, Yu Zhidan, Sun Huiqing

机构信息

Henan International Joint Laboratory of Children's Infectious Diseases, Department of Neonatology, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, China.

Department of Respiratory, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, China.

出版信息

Indian J Med Microbiol. 2023 May-Jun;43:11-17. doi: 10.1016/j.ijmmb.2022.09.012. Epub 2022 Oct 11.

DOI:10.1016/j.ijmmb.2022.09.012

PMID:36241528

Abstract

PURPOSE

The study was conducted to analyze the role of respiratory microbiome composition in children pneumonia etiology diagnosis.

METHODS

The bronchoalveolar lavage fluid bacterial community between the Mycoplasma pneumoniae pneumonia (MP group, n = 13) and the pathogen negative pneumonia (N group, n = 20) children were compared using the full-length 16S rRNA gene sequencing.

RESULTS

Distinct bacterial communities were identified in two groups and lower α-diversity was revealed in the MP patients indicating the lower abundance microbiota composition. Dominant bacteria were Mycoplasma and Mycoplasma pneumoniae for MP patients at genus and species levels. Possible pathogens were characterized in 17 out of 20 patients in the N group by detection of higher abundance using the 16S rRNA gene sequencing.

CONCLUSIONS

The high taxonomic resolution of full-length 16S rRNA gene sequencing assists in improving characterization of potential pathogens, and more studies are necessary to further evaluate the prognostic roles of specific bacteria in the pathogenicity of pneumonia.

摘要