胰岛素样生长因子-1诱导的肌肉源性干细胞作为雌性大鼠压力性尿失禁的一种潜在治疗方法

IGF-1-induced Muscle-Derived Stem Cells as a Potential Treatment of Stress Urinary Incontinence in Female Rats.

作者信息

Chen Chunjing, Xu Ying, Song Yanfeng

机构信息

Fuzong Clinical Medical College of Fujian Medical University, Fuzhou, Fujian 350025, China.

Fujian Provincial Maternity and Children's Hospital, affiliated hospital of Fujian Medical University, Fuzhou, Fujian 350005, China.

出版信息

Urol J. 2022 Dec 6;19(6):451-459. doi: 10.22037/uj.v19i.7066.

DOI:10.22037/uj.v19i.7066

PMID:36242527

Abstract

PURPOSE

Stress urinary incontinence (SUI) is prevalent among elderly women. This study aimed to discuss the potential of muscle-derived stem cells (MDSCs)-based therapy in treating SUI by exploring the effect of Insulin-like growth factor-1 (IGF-1) on transplanted MDSC and urethral sphincter function.

MATERIALS AND METHODS

Bilaterally pudendal nerve-transected (PNT) female rats were divided into four groups: sham, PNT+ phosphate buffered solution (PBS) injection, PNT+IGF-1/MDSCs and PNT+ green fluorescent protein (GFP)/MDSCs. IGF-1 was expressed in MDSCs by lentiviral vector. Viable MDSCs were detected by laser scanning confocal microscopy (LSCM). The expression of Myosin heavy chain (MyHC), vascular endothelial growth factor (VEGF), VEGF receptor 2 (VEGFR-2), microvessel density (MID) and urethral resistance function were assessed.

RESULTS

IGF-1 promoted the survival and differentiation of MDSCs. IGF-1-expressing MDSCs facilitated local angiogenesis and muscle fiber regeneration, and alleviated symptoms of SUI.

CONCLUSIONS

IGF-1-expressing MDSCs may be used as a novel treatment for patients with SUI.

摘要

目的

压力性尿失禁（SUI）在老年女性中普遍存在。本研究旨在通过探讨胰岛素样生长因子-1（IGF-1）对移植的肌肉源性干细胞（MDSCs）和尿道括约肌功能的影响，讨论基于MDSCs的治疗方法在治疗SUI中的潜力。

材料与方法

将双侧阴部神经切断（PNT）的雌性大鼠分为四组：假手术组、PNT+磷酸盐缓冲溶液（PBS）注射组、PNT+IGF-1/MDSCs组和PNT+绿色荧光蛋白（GFP）/MDSCs组。通过慢病毒载体在MDSCs中表达IGF-1。用激光扫描共聚焦显微镜（LSCM）检测存活的MDSCs。评估肌球蛋白重链（MyHC）,血管内皮生长因子（VEGF）、VEGF受体2（VEGFR-2）的表达、微血管密度（MID）和尿道阻力功能。