Human neutrophil antigen-1, -3, -4, and -5 allele and genotype frequencies in the Croatian blood donor population and their clinical significance.

OBJECTIVES

Human neutrophil antigens (HNAs) and antibodies play an important role in allo- and autoimmunity associated with immune neutropenia and transfusion reactions. The aim of this study was to determine the HNA-1, -3, -4 and -5 allele and genotype frequencies in the Croatian blood donor population to assess the role of HNA-1, -3, -4, and -5 alleles in the development of neonatal alloimmune neutropenia and antibody-mediated transfusion-related acute lung injury.

MATERIAL AND METHODS

A total of 371 blood samples from unselected healthy blood donors were analyzed. Samples from all 371 donors were genotyped for HNA-1, samples from 160 donors were genotyped for HNA-3, and samples from 142 donors were genotyped for HNA-4 and HNA-5 using the polymerase chain reaction with sequence-specific primers (PCR-SSP) method.

RESULTS

The frequencies of the FCGR3B01, FCGR3B02 and FCGR3B03 HNA-1 alleles were 0.393, 0.607 and 0.022, and of the SLC44A201 and SLC44A202 HNA-3 alleles 0.781 and 0.219, respectively. The frequencies of the ITGAM01 and ITGAM02 HNA-4 alleles were 0.796 and 0.204, and of the ITGAL01 and ITGAL*02 HNA-5 alleles 0.718 and 0.282, respectively.

CONCLUSION

These are the first results on the HNA allele and genotype frequencies in the Croatian blood donor population. We observed no deviations from previous reports on Caucasian populations. Determination of the HNA antigen frequencies in the population is important to estimate the risk of alloimmunization to HNA, especially the risk of fetal-maternal incompatibility and alloantibody production by transfusion of the HNA incompatible blood components.