在哈萨克斯坦东南部的库蠓属（Culicoides spp.）和临床健康的牲畜中鉴定和描述蓝舌病病毒。
Identification and characterization of bluetongue virus in Culicoides spp. and clinically healthy livestock in southeastern Kazakhstan.
机构信息
Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan.
Almaty Branch of the National Center for Biotechnology, Almaty 050054, Kazakhstan; M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan.
出版信息
Comp Immunol Microbiol Infect Dis. 2022 Nov-Dec;90-91:101895. doi: 10.1016/j.cimid.2022.101895. Epub 2022 Oct 7.
OBJECTIVE
Bluetongue is an arthropod-borne disease of ruminants. Here, we investigated the seroprevalence of bluetongue virus (BTV) in livestock and performed the first genetic characterization of BTV isolated from sheep and Culicoides midges in the southeastern region of Kazakhstan.
METHODS
Blood samples were collected from 1241 asymptomatic livestock. In addition, 497 Culicoides midges were collected. Samples were analyzed for specific anti-BTV antibodies and BTV RNA by ELISA and conventional RT-PCR, respectively.
RESULTS
The overall seroprevalence of BTV antibodies was shown to be 4.3 % (46/1079) in small ruminant and 1 % (1/82) in cattle. Anti-BTV antibodies were not detected in camels (0/80). The minimum infection rate of BTV in Culicoides was shown to be 0.24 %. Seg-2 and Seg-10 sequence analysis demonstrated that all isolates belonged to the 'western' topotype of the BTV-9 strain.
CONCLUSION
The present data confirm circulation of BTV in southeastern Kazakhstan.
目的
蓝舌病是一种反刍动物的节肢动物传播疾病。在这里，我们调查了哈萨克斯坦东南部牲畜中蓝舌病病毒（BTV）的血清流行率，并对从绵羊和虻属虻中分离到的 BTV 进行了首次遗传特征分析。
方法
采集了 1241 份无症状牲畜的血液样本。此外，还采集了 497 只虻属虻。通过酶联免疫吸附试验（ELISA）和常规 RT-PCR 分别对样本进行了针对特定 BTV 抗体和 BTV RNA 的分析。
结果
小反刍动物的 BTV 抗体总血清阳性率为 4.3%（46/1079），牛为 1%（1/82）。骆驼（0/80）未检测到抗 BTV 抗体。虻属虻中的 BTV 最小感染率为 0.24%。Seg-2 和 Seg-10 序列分析表明，所有分离株均属于 BTV-9 株的“西部”拓扑型。
结论
本数据证实了 BTV 在哈萨克斯坦东南部的传播。
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