pH 值和单价/二价阳离子对银鲫肌原纤维蛋白结构和理化性质的影响。

The influence of pH and monovalent/divalent cations on the structural and physicochemical properties of myofibrillar protein from silver carp.

机构信息

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan, Hubei 430070, China; National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Wuhan, Hubei 430070, China; Aquatic Product Engineering and Technology Research Center of Hubei Province, Wuhan, Hubei 430070, China.

出版信息

Food Chem. 2023 Mar 15;404(Pt A):134519. doi: 10.1016/j.foodchem.2022.134519. Epub 2022 Oct 5.

DOI:10.1016/j.foodchem.2022.134519

PMID:36252377

Abstract

The present study aimed to investigate effects of pH and monovalent (Na and K)/divalent (Ca and Mg) cations on the structural and physicochemical properties of myofibrillar protein (MP) from silver carp. MP treated with divalent cation had lesser change for the structure than that treated with monovalent cation. Ca-ATPase activity of MP treated with monovalent cation was increased firstly and then decreased, while that treated with divalent cation was decreased with increasing ionic strength. Surface hydrophobicity and Z-average of MP treated with divalent cations was lower than that with monovalent cations, while they decreased and then increased with the pH shifting from 3.0 to 9.0. Zeta potential of MP was increased and then decreased with increasing the pH but decreased and then increased with increasing ionic strength. In general, the pH and monovalent/divalent cations could cause various hydrophobic and electrostatic interactions, resulting in changes of the physicochemical properties of MP.

摘要

本研究旨在探讨 pH 值以及单价（Na 和 K）/二价（Ca 和 Mg）阳离子对银鲫肌原纤维蛋白（MP）结构和理化特性的影响。与单价阳离子处理相比，二价阳离子处理的 MP 结构变化较小。用单价阳离子处理的 MP 的 Ca-ATPase 活性先增加后减少，而用二价阳离子处理的 MP 的 Ca-ATPase 活性则随着离子强度的增加而减少。用二价阳离子处理的 MP 的表面疏水性和 Z-平均粒径低于用单价阳离子处理的 MP，而随着 pH 值从 3.0 升高到 9.0，它们先降低后升高。用二价阳离子处理的 MP 的 Zeta 电位先升高后降低，而用单价阳离子处理的 MP 的 Zeta 电位则先升高后降低，再升高。一般来说，pH 值和单价/二价阳离子会引起各种疏水和静电相互作用，导致 MP 理化特性的变化。