监测细胞内活性氧物种水平对组蛋白去乙酰化酶抑制剂的反应变化。
Monitoring Changes in Intracellular Reactive Oxygen Species Levels in Response to Histone Deacetylase Inhibitors.
机构信息
Institute of Toxicology, University Medical Center Mainz, Mainz, Germany.
Department of Zoology, Faculty of Science, Aswan University, Aswan, Egypt.
出版信息
Methods Mol Biol. 2023;2589:337-344. doi: 10.1007/978-1-0716-2788-4_22.
PMID:36255635
Abstract
Reactive oxygen species (ROS) are induced by several chemotherapeutics. In this protocol, we describe a flow cytometry-based method for the analysis of the intracellular levels of ROS in vital leukemic cells in response to the histone deacetylase inhibitor vorinostat. This measurement of ROS using the cell-permeable dye CM-H2DCFDA indicates intracellular oxidative stress.
摘要
活性氧 (ROS) 可被多种化疗药物诱导产生。在本方案中，我们描述了一种基于流式细胞术的方法，用于分析组蛋白去乙酰化酶抑制剂伏立诺他作用下活白血病细胞内 ROS 水平。使用细胞通透性染料 CM-H2DCFDA 测量 ROS 可指示细胞内氧化应激。
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