Xie Shanshan, Guan Changbiao, Huang Tingmo, Liu Yuqian, Yuan Feifei, Xu Daqi
Department of Sports Medicine, Xiangya Hospital, Central South University, Changsha, China.
Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, China.
J Orthop Translat. 2022 Oct 13;36:216-224. doi: 10.1016/j.jot.2022.09.006. eCollection 2022 Sep.
The repair of rotator cuff injury is affected by lifestyle and metabolic factors. Intermittent fasting (IF) can promote repair of damaged tissue by regulating intestinal flora, which provides an idea of therapy for rotator cuff injury. The aim of this study was to investigate the effects of fasting on rotator cuff repair after injury, and the role of intestinal flora or a single strain in this process.
Mice underwent rotator cuff injury were treated with intermittent fasting or fed . Fasting began one month before surgery and continued until euthanasia. Fresh feces were collected at 2 weeks before surgery, on the day of surgery, and 2, 4, 8 weeks postoperatively for 16S rRNA microbiome sequencing. Supraspinatus tendon-humerus (SSTH) complex was collected at 2, 4 and 8 weeks after surgery. Live () was used for repair of rotator cuff injury, with equal amount of pasteurized (KPD) or sterile anaerobic phosphate buffer saline (PBS) as control. Biomechanical, radiological, histological analysis were used to assess the effect of rotator cuff repair.
Biomechanical, radiological and histological analysis indicated that intermittent fasting significantly promoted the repair of rotator cuff injury in the early postoperative period ( ＜ 0.05), but significantly inhibited the repair of rotator cuff injury at 4 weeks postoperatively ( ＜ 0.05). 16S rRNA Microbiome sequencing result showed that was the species with the most obvious changes in intestinal flora of mice after fasting. The results of tensile test, X-ray analysis and histological analysis indicated that the live (LPD) significantly impaired the biomechanical properties, bone regeneration and fibrocartilage regeneration of enthesis postoperatively ( ＜ 0.05).
Intermittent fasting promoted repair of rotator cuff injury in the early postoperative period by regulating the gut microbiota, in which played an important role.
Intermittent fasting (IF) may be a beneficial lifestyle for the repair of rotator cuff injury in the early postoperative period in clinical, and the influence of a certain strain on the repair of rotator cuff injury may also provide an idea for the treatment of rotator cuff injury in the future.
肩袖损伤的修复受生活方式和代谢因素影响。间歇性禁食（IF）可通过调节肠道菌群促进受损组织修复，这为肩袖损伤的治疗提供了思路。本研究旨在探讨禁食对损伤后肩袖修复的影响，以及肠道菌群或单一菌株在此过程中的作用。
对接受肩袖损伤的小鼠进行间歇性禁食或正常喂食。禁食在手术前1个月开始并持续至安乐死。在手术前2周、手术当天以及术后2、4、8周收集新鲜粪便进行16S rRNA微生物组测序。在术后2、4和8周收集冈上肌腱 - 肱骨（SSTH）复合体。将活的（）用于肩袖损伤修复，等量的巴氏杀菌的（KPD）或无菌厌氧磷酸盐缓冲盐水（PBS）作为对照。采用生物力学、放射学、组织学分析评估肩袖修复效果。
生物力学、放射学和组织学分析表明，间歇性禁食在术后早期显著促进了肩袖损伤的修复（＜0.05），但在术后4周显著抑制了肩袖损伤的修复（＜0.05）。16S rRNA微生物组测序结果显示，是禁食后小鼠肠道菌群中变化最明显的物种。拉伸试验、X射线分析和组织学分析结果表明，活的（LPD）术后显著损害了附着点的生物力学性能、骨再生和纤维软骨再生（＜0.05）。
间歇性禁食通过调节肠道微生物群促进了术后早期肩袖损伤的修复，其中起重要作用。
间歇性禁食（IF）在临床上可能是术后早期肩袖损伤修复的有益生活方式，特定菌株对肩袖损伤修复的影响也可能为未来肩袖损伤的治疗提供思路。
