SRRM1 通过调控 JAK/STAT 信号通路促进肝癌细胞的增殖、迁移和侵袭。
SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway.
机构信息
Interventional Oncology Department, Jilin Medical College Affiliated Hospital, Jilin 132003, China.
Cardiothoracic Interventional Vascular Surgery, Chengde Central Hospital, Chengde, Hebei 067000, China.
出版信息
Tissue Cell. 2022 Dec;79:101954. doi: 10.1016/j.tice.2022.101954. Epub 2022 Oct 12.
BACKGROUND
To explore the regulatory effect of serine/arginine repetitive matrix 1 (SRRM1) on hepatocellular carcinoma (HCC) and its potential pathway.
METHODS
SRRM1 level in the tissue was tested by western blot and immunohistochemistry. Cells proliferation, apoptosis, migration, and invasion were tested by EdU, flow cytometry, wound healing assay, and Transwell, respectively. The potential mechanism of SRRM1 was explored through GSEA enrichment analysis and GeneMANIA protein-protein interaction (PPI) network. Co-immunoprecipitation assay was used to detect PPI. Levels of Bcl-2, Bax, Cleaved caspase 3, E-cadherin, N-cadherin, Vimentin, p-JAK2, JAK2, p-STAT3, and STAT3 were tested by Western blot. SRRM1 was highly expressed in HCC tissues, and was related to the survival and prognosis of patients.
RESULTS
SRRM1 is significantly upregulated in HCC tumour tissues and correlated with progression of HCC. Overexpression of SRRM1 accelerated the proliferation, migration and invasion of HCC cells, and inhibited cell apoptosis, but low expression of SRRM1 had the opposite effect. SRRM1 positively correlated with the expression of IL6ST (GP130) and activated the JAK/STAT signaling pathways. SRRM1 affected the level of key molecules p-JAK2, JAK2, p-STAT3, and STAT3 in the JAK/STAT pathway.
CONCLUSION
SRRM1 played a significant role in the proliferation, migration, invasion and apoptosis of HCC, and promoted cancer by regulating the JAK/STAT signaling pathway.
背景
探讨丝氨酸/精氨酸重复矩阵蛋白 1（SRRM1）对肝细胞癌（HCC）的调节作用及其潜在机制。
方法
采用 Western blot 和免疫组化检测组织中 SRRM1 水平。通过 EdU、流式细胞术、划痕愈合实验和 Transwell 分别检测细胞增殖、凋亡、迁移和侵袭。通过 GSEA 富集分析和 GeneMANIA 蛋白质-蛋白质相互作用（PPI）网络探索 SRRM1 的潜在机制。采用 co-immunoprecipitation 检测 PPI。Western blot 检测 Bcl-2、Bax、Cleaved caspase 3、E-cadherin、N-cadherin、Vimentin、p-JAK2、JAK2、p-STAT3 和 STAT3 的水平。
结果
SRRM1 在 HCC 肿瘤组织中显著上调，与 HCC 的进展相关。SRRM1 的过表达加速了 HCC 细胞的增殖、迁移和侵袭，抑制了细胞凋亡，而 SRRM1 的低表达则产生相反的效果。SRRM1 与 IL6ST（GP130）的表达呈正相关，并激活了 JAK/STAT 信号通路。SRRM1 影响 JAK/STAT 通路中关键分子 p-JAK2、JAK2、p-STAT3 和 STAT3 的水平。
结论
SRRM1 在 HCC 的增殖、迁移、侵袭和凋亡中发挥重要作用，通过调节 JAK/STAT 信号通路促进癌症的发生。
Zotero 插件