Odum School of Ecology, The University of Georgia, Athens, GA, USA.
D.B. Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2023;2562:443-458. doi: 10.1007/978-1-0716-2659-7_29.
Assessing numbers of leukocytes in salamanders and other amphibians can be useful metrics for understanding health or stress levels of individuals in a population. In this chapter we describe the procedures for obtaining blood samples from amphibians, preparing blood films for microscopy, counting, and identifying cells. We also provide reference values for amphibian leukocytes for use in interpreting leukocyte data. From our assessment of the published and unpublished literature, "non-stressed" salamanders would have a leukocyte profile where 60-70% of cells are lymphocytes, 17-30% are neutrophils, 1-4% are eosinophils, 4-12% are basophils, and 2-6% are monocytes. In Ambystoma spp., the eosinophil abundance can be notably higher (30% of all white blood cells), for reasons unknown. Finally, the neutrophil-lymphocyte ratio of most non-stressed salamanders tends to be between 0.3 and 0.4 (sometimes less), while the ratios of stressed salamanders tend to be over 1.0.
评估蝾螈和其他两栖动物的白细胞数量可以作为了解个体在种群中的健康或应激水平的有用指标。在本章中，我们将描述从两栖动物中采集血液样本、准备血液涂片进行显微镜检查、计数和识别细胞的程序。我们还提供了用于解释白细胞数据的两栖动物白细胞的参考值。根据我们对已发表和未发表文献的评估，“无应激”的蝾螈的白细胞谱中，60-70%的细胞为淋巴细胞，17-30%为中性粒细胞，1-4%为嗜酸性粒细胞，4-12%为嗜碱性粒细胞，2-6%为单核细胞。在Ambystoma spp.中，嗜酸性粒细胞的丰度可能明显更高（占所有白细胞的 30%），原因不明。最后，大多数无应激蝾螈的中性粒细胞与淋巴细胞比值在 0.3 到 0.4 之间（有时更低），而应激蝾螈的比值往往超过 1.0。
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