骨不连中 lncRNA 和 mRNA 表达谱的转录组测序分析
Transcriptome Sequencing Analysis of lncRNA and mRNA Expression Profiles in Bone Nonunion.
机构信息
Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
Department of Respiratory Medicine, National Key Clinical Specialty, Branch of National Clinical Research Center for Respiratory Disease, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
出版信息
Oxid Med Cell Longev. 2022 Oct 12;2022:9110449. doi: 10.1155/2022/9110449. eCollection 2022.
BACKGROUND
Bone nonunion is a serious complication of fracture. This study explored the differentially expressed lncRNAs (DELs) and mRNAs (DEGs) and identified potential lncRNA-mRNA interactions in bone nonunion.
METHODS
We extracted total RNA from three bone nonunion and three bone union patient tissue samples. RNA sequencing was performed to detect DELs and DEGs between bone nonunion and union tissue samples. The lncRNAs and genes with absolute log2-fold change (log2FC) > 1 and adjusted value < 0.05 were further chosen for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. lncRNA and targeted mRNA interaction networks were constructed.
RESULTS
We observed 179 DELs and 415 DEGs between the bone nonunion and union tissue samples. GO analysis indicated that DELs and DEGs were mainly enriched in the chondroitin sulfate proteoglycan biosynthetic process. DELs and DEGs were enriched in "ECM-receptor interaction" and " infection" KEGG pathways. Several potential lncRNA-mRNA interactions were also predicted.
CONCLUSIONS
This study identified bone nonunion-associated lncRNAs and mRNAs using deep sequencing that may be useful as potential biomarkers for bone nonunion.
背景
骨不连是骨折的一种严重并发症。本研究通过探索差异表达的长链非编码 RNA（lncRNAs）（DELs）和信使 RNA（mRNAs）（DEGs），并鉴定骨不连中的潜在 lncRNA-mRNA 相互作用。
方法
我们从三例骨不连和三例骨愈合患者的组织样本中提取总 RNA。通过 RNA 测序检测骨不连和骨愈合组织样本之间的 DELs 和 DEGs。进一步选择具有绝对对数倍变化（log2FC）> 1 和调整 值 < 0.05 的 lncRNAs 和基因进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析。构建 lncRNA 和靶向 mRNA 相互作用网络。
结果
我们观察到骨不连和骨愈合组织样本之间有 179 个 DELs 和 415 个 DEGs。GO 分析表明，DELs 和 DEGs 主要富集在软骨素硫酸蛋白聚糖生物合成过程中。DELs 和 DEGs 富集在“ECM-受体相互作用”和“感染”KEGG 途径中。还预测了几个潜在的 lncRNA-mRNA 相互作用。
结论
本研究通过深度测序鉴定了与骨不连相关的 lncRNAs 和 mRNAs，它们可能作为骨不连的潜在生物标志物。
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