Speth R C, Dinh T T, Ritter S
Peptides. 1987 Jul-Aug;8(4):677-85. doi: 10.1016/0196-9781(87)90043-x.
Angiotensin II (Ang II) receptor binding sites in the dorsomedial medulla of intact and unilaterally nodose ganglionectomized rats were identified and characterized using 125I-sarcosine,isoleucine Ang II. This radioligand bound saturably and with high affinity to rat brain homogenates and to sections of rat brainstem. Specific (1 microM angiotensin II displaceable) binding of 125I-sarcosine,isoleucine Ang II was displaced by angiotensin analogues with a potency order similar to that described for angiotensin II receptors. Unilateral nodose ganglionectomy caused a reduction in Ang II receptor binding in the medial solitary tract nucleus, dorsal motor nucleus of the vagus, and area postrema ipsilateral to the lesioned ganglion. This observation suggests that Ang II receptors in the dorsomedial medulla may be located on axon terminals of vagal afferents and cell bodies of vagal efferents.
利用¹²⁵I-肌氨酸、异亮氨酸血管紧张素II，对完整和单侧结状神经节切除大鼠延髓背内侧的血管紧张素II（Ang II）受体结合位点进行了鉴定和表征。这种放射性配体与大鼠脑匀浆和大鼠脑干切片以饱和且高亲和力的方式结合。¹²⁵I-肌氨酸、异亮氨酸血管紧张素II的特异性（1微摩尔血管紧张素II可置换）结合可被血管紧张素类似物置换，其效力顺序与血管紧张素II受体所描述的相似。单侧结状神经节切除导致损伤神经节同侧的孤束核内侧、迷走神经背运动核和最后区的Ang II受体结合减少。这一观察结果表明，延髓背内侧的Ang II受体可能位于迷走神经传入纤维的轴突终末和迷走神经传出纤维的细胞体上。
