建立并鉴定人肾透明细胞肉瘤和恶性黑色素瘤细胞系。

Establishment and Characterisation of Human Clear Cell Sarcoma and Malignant Melanoma Cell Lines.

机构信息

Department of Pathology, Fukuoka University Hospital, Fukuoka, Japan;

Department of Pathology, Fukuoka University Hospital, Fukuoka, Japan.

出版信息

Anticancer Res. 2022 Nov;42(11):5315-5322. doi: 10.21873/anticanres.16038.

DOI:10.21873/anticanres.16038

PMID:36288876

Abstract

BACKGROUND/AIM: Clear cell sarcoma of soft tissue (CCSST) and conventional malignant melanoma (MM) are rare and aggressive tumours with similarities in morphology and the expression of melanocytic markers.

MATERIALS AND METHODS

We established two CCSST cell lines (FU-CCSST-1 and FU-CCSST-2) from soft tissues of the patella and supraclavicular. A MM cell line (FU-MM-1) was established from lymph node metastases of subungual malignant melanoma.

RESULTS

FU-CCSST-2 cells were transplantable to immunodeficient mice. Immunohistochemical studies demonstrated tumour cells were negative for cytokeratin AE1/AE3 and positive for S100 protein, HMB45, Melan-A, CD146 and SOX10 in all specimens. FU-CCSST-1 and FU-CCSST-2 harboured t(12;22)(q13;q12) translocations with expression of the EWSR1/ATF1 fusion gene. FU-MM-1 demonstrated loss of the short arm of chromosome 9 and harboured wild-type BRAF (codon 469 and 600) and NRAS (codon 12, 13 and 61).

CONCLUSION

We report the establishment and characterisation of CCSST and MM cell lines that may have utility in the study of pathogenic mechanisms and development of novel therapeutic reagents.

摘要

背景/目的：软组织透明细胞肉瘤（CCSST）和传统恶性黑色素瘤（MM）是罕见且侵袭性的肿瘤，在形态学和黑色素细胞标志物表达上具有相似性。

材料和方法

我们从髌骨和锁骨上软组织中建立了两个 CCSST 细胞系（FU-CCSST-1 和 FU-CCSST-2）。从甲下恶性黑色素瘤的淋巴结转移中建立了一个 MM 细胞系（FU-MM-1）。

结果

FU-CCSST-2 细胞可移植到免疫缺陷小鼠中。免疫组织化学研究表明，所有标本中的肿瘤细胞均对细胞角蛋白 AE1/AE3 呈阴性，而对 S100 蛋白、HMB45、Melan-A、CD146 和 SOX10 呈阳性。FU-CCSST-1 和 FU-CCSST-2 均存在 t(12;22)(q13;q12) 易位，表达 EWSR1/ATF1 融合基因。FU-MM-1 显示 9 号染色体短臂缺失，并存在野生型 BRAF（密码子 469 和 600）和 NRAS（密码子 12、13 和 61）。