Buendía Débora, Guncay Tatiana, Oyanedel Macarena, Lemus Makarena, Weinstein Alejandro, Ardiles Álvaro O, Marcos José, Fernandes Adriana, Zângaro Renato, Muñoz Pablo
Programa de Engenharia Biomédica, Instituto de Engenharia Biomédica, Universidade Anhembi Morumbi-UAM, Rua Casa do Ator, 294, Sao Paulo 04546-001, Brazil.
Escuela de Ingeniería Civil Biomédica, Facultad de Ingeniería, Universidad de Valparaíso, General Cruz 222, Valparaíso 2362905, Chile.
Brain Sci. 2022 Sep 21;12(10):1272. doi: 10.3390/brainsci12101272.
Alzheimer's disease (AD) is the main cause of dementia worldwide. Emerging non-invasive treatments such as photobiomodulation target the mitochondria to minimize brain damage, improving cognitive functions. In this work, an experimental design was carried out to evaluate the effect of transcranial light therapy (TLTC) on synaptic plasticity (SP) and cognitive functions in an AD animal model. Twenty-three mice were separated into two general groups: an APP/PS1 (ALZ) transgenic group and a wild-type (WT) group. Each group was randomly subdivided into two subgroups: mice with and without TLTC, depending on whether they would undergo treatment with TLTC. Cognitive function, measured through an object recognition task, showed non-significant improvement after TLTC. SP, on the other hand, was evaluated using four electrophysiological parameters from the Schaffer-CA1 collateral hippocampal synapses: excitatory field potentials (fEPSP), paired pulse facilitation (PPF), long-term depression (LTD), and long-term potentiation (LTP). An improvement was observed in subjects treated with TLTC, showing higher levels of LTP than those transgenic mice that were not exposed to the treatment. Therefore, the results obtained in this work showed that TLTC could be an efficient non-invasive treatment for AD-associated SP deficits.
阿尔茨海默病（AD）是全球痴呆症的主要病因。诸如光生物调节等新兴的非侵入性治疗方法以线粒体为靶点，将脑损伤降至最低，改善认知功能。在这项研究中，进行了一项实验设计，以评估经颅光疗法（TLTC）对AD动物模型中突触可塑性（SP）和认知功能的影响。23只小鼠被分为两个总体组：APP/PS1（ALZ）转基因组和野生型（WT）组。根据是否接受TLTC治疗，每组又随机细分为两个亚组：接受TLTC治疗的小鼠和未接受TLTC治疗的小鼠。通过物体识别任务测量的认知功能在TLTC治疗后没有显著改善。另一方面，使用来自海马体Schaffer-CA1侧支突触的四个电生理参数评估SP：兴奋性场电位（fEPSP）、双脉冲易化（PPF）、长时程抑制（LTD）和长时程增强（LTP）。在接受TLTC治疗的小鼠中观察到了改善，其LTP水平高于未接受该治疗的转基因小鼠。因此，这项研究获得的结果表明，TLTC可能是一种治疗与AD相关的SP缺陷的有效非侵入性疗法。
