Fisseha Nebeyi, Hammeso Workineh Woldeselassie, Nureye Dejen
Department of Pharmacy, School of Pharmacy, College of Health Sciences, Mizan-Tepi University, Mizan-Aman, South West, Ethiopia.
J Exp Pharmacol. 2022 Oct 20;14:291-299. doi: 10.2147/JEP.S374890. eCollection 2022.
Scientists and researchers continue to focus on medicinal plants as a potential source of lead chemicals in the search for and development of new antiepileptic medicines. Welw. Syn is used to treat epilepsy in Ethiopian traditional medicine. The anticonvulsant effect of Welw. Syn hydroalcoholic extract and solvent fractions was evaluated in this study since the claim has not been thoroughly explored.
The plant's root was extracted using the maceration procedure, with aqueous, butanol, and chloroform as solvents. The maximum electroshock and pentylenetetrazol model tests were used to assess anticonvulsant activity. Mice were divided into five groups (n = 6) at random. The test groups received 100, 200, and 400 mg/kg of hydroalcoholic extract and solvent fraction, respectively. For the maximum electric shock test, the positive control groups received 25 mg/kg phenytoin and 200 mg/kg valproate for the pentylenetetrazol test. The negative control was given 10 mL/kg of pure water or 2% Tween 80.
There were no signs of toxicity in the hydroalcoholic extract or solvent extraction. When compared to the negative control, the hydro-alcoholic extract had a significant anticonvulsant effect in both the maximum electric shock test and the pentylenetetrazol test. In both cases, the butanol component had a comparable impact. In the pentylenetetrazol test, the chloroform fraction had a significant anticonvulsant effect when compared to the control at dosages of MB200 and MB400. Flavonoids, phenols, tannins, steroids, terpenoids, and saponins were found in both the hydroalcoholic and solvent fractions of the plant extract.
The plant appears to have promising anticonvulsant properties, and it might be used to generate novel anti-epileptic drugs, according to this study.
在寻找和开发新型抗癫痫药物的过程中，科学家和研究人员持续将药用植物视为潜在的先导化合物来源。在埃塞俄比亚传统医学中，惠氏合瓣花（Welw. Syn）被用于治疗癫痫。由于这一说法尚未得到充分探究，本研究对惠氏合瓣花水醇提取物及溶剂萃取物的抗惊厥作用进行了评估。
采用浸渍法，以水、丁醇和氯仿为溶剂对该植物的根进行提取。通过最大电休克和戊四氮模型试验评估抗惊厥活性。将小鼠随机分为五组（每组n = 6）。试验组分别给予100、200和400 mg/kg的水醇提取物及溶剂萃取物。在最大电休克试验中，阳性对照组给予25 mg/kg苯妥英钠；在戊四氮试验中，阳性对照组给予200 mg/kg丙戊酸。阴性对照组给予10 mL/kg纯水或2%吐温80。
水醇提取物或溶剂萃取物均未显示出毒性迹象。与阴性对照组相比，水醇提取物在最大电休克试验和戊四氮试验中均具有显著的抗惊厥作用。在这两种情况下，丁醇成分都有类似的效果。在戊四氮试验中，氯仿萃取物在MB200和MB400剂量下与对照组相比具有显著的抗惊厥作用。在植物提取物的水醇提取物和溶剂萃取物中均发现了黄酮类、酚类、单宁、甾体、萜类和皂苷。
根据本研究，该植物似乎具有良好的抗惊厥特性，可能用于开发新型抗癫痫药物。
