Cheng Zhimei, Yang Qin, He Huizhou, Li Ran, Li Xueying, Jiang Hongyu, Zhao Xuya, Li Junxiang, Wang Lizhou, Zhou Shi, Zhang Shuai
Institute of Image, Guizhou Medical University, Guiyang, China.
GCP Institution Office, The Affiliated Cancer Hospital of Guizhou Medical University, Guiyang, China.
Front Physiol. 2022 Oct 12;13:893925. doi: 10.3389/fphys.2022.893925. eCollection 2022.
In this study, we sought to evaluate changes in peritumoral fibrosis after transarterial chemoembolization (TACE) in a rabbit VX2 liver tumor model using intravoxel incoherent motion diffusion-weighted imaging (IVIM DWI) and ultrasound shear wave elastography (SWE). A total of 20 rabbits underwent implantation of VX2 tumor tissues in the left lobe of the liver. The rabbits were randomly divided into an experimental group ( = 10) or a control group ( = 10). Those in the experimental group were treated with an emulsion of lipiodol and pirarubicin through a microcatheter 2-3 weeks after implantation; those in the control group were treated with sterile water. Compared with the control group, the true diffusion coefficient (D) and pseudodiffusion coefficient (D*) values in liver tissues were significantly lower (p < 0.05 for all) and liver stiffness values (LSV) (10.58 ± 0.89 kPa) were higher in the experimental group (7.65 ± 0.86 kPa; p < 0.001). The median stage of liver fibrosis based on METAVIR scores was 1 (1,1) in the control group and 2 (2,3) in the experimental group ( = 4.15, p < 0.001). D, D*, and LSV were significantly correlated with pathologic staining in the assessment of liver fibrosis ( = -0.54 p = 0.015; = -0.50, p = 0.025; = 0.91, p < 0.001; respectively). These data suggest that TACE aggravates liver injury and liver fibrosis, especially surrounding the tumor, in a rabbit VX2 liver tumor model. IVIM DWI and SWE can be used to evaluate the change in liver fibrosis.
在本研究中，我们试图在兔VX2肝肿瘤模型中，使用体素内不相干运动扩散加权成像（IVIM DWI）和超声剪切波弹性成像（SWE）来评估经动脉化疗栓塞（TACE）后肿瘤周围纤维化的变化。总共20只兔在肝脏左叶植入VX2肿瘤组织。将兔随机分为实验组（n = 10）或对照组（n = 10）。实验组在植入后2 - 3周通过微导管用碘油和吡柔比星乳剂治疗；对照组用无菌水治疗。与对照组相比，实验组肝组织中的真实扩散系数（D）和伪扩散系数（D*）值显著更低（均p < 0.05），肝硬度值（LSV）更高（实验组为10.58±0.89 kPa，对照组为7.65±0.86 kPa；p < 0.001）。基于METAVIR评分的肝纤维化中位阶段在对照组为1（1,1），在实验组为2（2,3）（χ² = 4.15，p < 0.001）。在肝纤维化评估中，D、D*和LSV与病理染色显著相关（分别为r = -0.54，p = 0.015；r = -0.50，p = 0.025；r = 0.91，p < 0.001）。这些数据表明，在兔VX2肝肿瘤模型中，TACE会加重肝损伤和肝纤维化，尤其是肿瘤周围的肝纤维化。IVIM DWI和SWE可用于评估肝纤维化的变化。
