Peng Keqin, Pan Yintao, Tan Tingjun, Zeng Xiangyu, Lin Meiling, Jiang Shuang, Zhao Zhibo, Tian Fenghua, Zhao Xiaosheng
Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, China.
Front Microbiol. 2022 Sep 27;13:923181. doi: 10.3389/fmicb.2022.923181. eCollection 2022.
Sweet cherry is an important fruit crop with high economic and ornamental value in China. However, cherry fruit anthracnose, caused by species, greatly impacts cherry yield and quality. Here, we surveyed cherry anthracnose in Guizhou, China from 2019-2020. Necrotic sweet cherry fruits were collected from different areas in Guizhou and examined. A total of 116 strains were isolated from these symptomatic fruits. Based on the morphological characteristics of the isolates and phylogenetic analyses of concatenate internal transcribed spacer (ITS) region and , , , and genes, the pathogen responsible for causing sweet cherry anthracnose was identified as . Pathogenicity tests were conducted by inoculating healthy sweet cherry fruits with spore suspensions of the fungal pathogen, and Koch's postulates were confirmed by pathogen re-isolation and identification. The Q-1 isolate showed different sensitivities to 13 fungicides, exhibiting seven different modes of action, and its EC values ranged from 0.04 to 91.26 μg ml. According to that, the sensitivity of 20 isolates from different samples to ten fungicides with better performance, were measured. The results showed that 6 of the 10 fungicides (difenoconazole, propiconazole, prochloraz-manganese, pyraclostrobin, trifloxystrobin-tebuconazole, and difenoconazole-azoxystrobin) all showed higher sensitive to the 20 isolates, and no resistance groups appeared. Its EC50 values ranged from 0.013 to 1.563 μg ml. In summary, this is the first report demonstrating that causes sweet cherry anthracnose and the results of this study provide insights into how sweet cherry anthracnose could be effectively controlled in China.
甜樱桃是中国一种具有较高经济价值和观赏价值的重要水果作物。然而，由[具体物种]引起的樱桃果实炭疽病对樱桃的产量和品质有很大影响。在此，我们于2019年至2020年对中国贵州的樱桃炭疽病进行了调查。从贵州不同地区采集了坏死的甜樱桃果实并进行检查。共从这些有症状的果实中分离出116个菌株。基于分离株的形态特征以及对串联内转录间隔区（ITS）区域和[其他基因名称]、[其他基因名称]、[其他基因名称]和[其他基因名称]基因的系统发育分析，确定引起甜樱桃炭疽病的病原体为[具体病原体名称]。通过用真菌病原体的孢子悬浮液接种健康甜樱桃果实进行致病性测试，并通过病原体的重新分离和鉴定证实了科赫法则。Q - 1分离株对13种杀菌剂表现出不同的敏感性，呈现出七种不同的作用模式，其EC值范围为0.04至91.26μg/ml。据此，测定了来自不同样品的20个分离株对性能较好的十种杀菌剂的敏感性。结果表明，10种杀菌剂中的6种（苯醚甲环唑、丙环唑、咪鲜胺锰盐、吡唑醚菌酯、肟菌酯·戊唑醇和苯甲·嘧菌酯）对这20个分离株均表现出较高的敏感性，未出现抗性群体。其EC50值范围为0.013至1.563μg/ml。总之，这是首次报道[具体病原体名称]引起甜樱桃炭疽病，本研究结果为中国有效控制甜樱桃炭疽病提供了见解。
