Heng Pengfei, Liu Jiakai, Song Zhen, Wu Chuan, Yu Xiuzhong, He Yang
State Key Laboratory of Southwestern Chinese Medicine Resources, College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Department of Ultrasound Medicine, The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, Sichuan, China.
Front Microbiol. 2022 Oct 13;13:1027785. doi: 10.3389/fmicb.2022.1027785. eCollection 2022.
is a common pathogen that causes various infections. Therefore, it is crucial to develop a fast and easy detection method for diagnosing and preventing infections. In this study, MIRA assay was developed and validated (specificity; 100%) for the detection of with as the target gene. The reaction temperature and reaction time were then optimized, and the best reaction was at 40°C, 20 min. The assay could detect in only 25 min. Additionally, the limit of detection of MIRA was 5 × 10 CFU/ml, 10-fold lower than that of the traditional PCR. Furthermore, this assay efficiently detected 219 of 335 strains obtained from different bacterial samples (detection accuracy; 99.40%). In conclusion, this study provides a rapid and easy-to-operate method for the detection of , and thus can be used for the timely diagnosis and prevention of infection.
是一种引起各种感染的常见病原体。因此，开发一种快速简便的检测方法用于诊断和预防感染至关重要。在本研究中，开发并验证了MIRA检测法（特异性；100%）用于以 为靶基因检测 。然后优化了反应温度和反应时间，最佳反应条件为40°C、20分钟。该检测法仅需25分钟即可检测到 。此外，MIRA的检测限为5×10 CFU/ml，比传统PCR低10倍。此外，该检测法有效检测了从不同细菌样本中获得的335株菌株中的219株（检测准确率；99.40%）。总之，本研究提供了一种快速且易于操作的 检测方法，因此可用于及时诊断和预防 感染。
