Queiroz Marcela Borsatto, Inada Rafaela N H, Jampani José Leandro de Abreu, Guerreiro-Tanomaru Juliane Maria, Sasso-Cerri Estela, Tanomaru-Filho Mário, Cerri Paulo Sérgio
Department of Restorative Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
Laboratory of Histology and Embryology, Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, Dental School, São Paulo State University (UNESP), Araraquara, Brazil.
Int Endod J. 2023 Feb;56(2):259-277. doi: 10.1111/iej.13863. Epub 2022 Nov 13.
To evaluate the tissue reaction of a tricalcium silicate-based repair material associated with 30% calcium tungstate (TCS + CaWO ) in comparison to Bio-C Repair (Bio-C; Angelus) and to MTA Repair HP (MTA HP; Angelus).
Polyethylene tubes filled with one of the materials or left empty (control group, CG) were implanted into the subcutaneous tissues of rats for 7, 15, 30 and 60 days (n = 32/group). The capsule thickness, number of inflammatory cells, collagen content, interleukin-6 (IL-6), osteocalcin (OCN), von Kossa reaction and analysis under polarized light were evaluated. The data were subjected to generalized linear models for repeated measures, except the OCN. OCN data were submitted to Kruskal-Wallis and Dunn's post hoc test and Friedman followed by Nemenyi's test at significance level of 5%.
At all time points, significant differences in the number of inflammatory cells were not observed between TCS + CaWO and Bio-C, whereas, at 15, 30 and 60 days, no significant difference was detected between TCS + CaWO and MTA HP. At all periods, significant differences were not detected in the number of fibroblasts in TCS + CaWO versus MTA HP, and, at 60 days, no significant difference was demonstrated between these groups and CG. Significant differences in the immunoexpression of IL-6 were not detected amongst bioceramic materials at all periods. From 7 to 60 days, significant reduction in the number of inflammatory cells, number of IL-6-immunopositive cells and in the capsule thickness was accompanied by significant increase in the collagen in all groups. OCN-immunolabelled cells, von Kossa-positive structures and amorphous calcite deposits were observed around all materials, whereas, in the CG, these structures were not seen.
These findings indicate that the experimental material (TCS + CaWO ) is biocompatible and has a bioactive potential, similar to the MTA HP and Bio-C Repair, and suggest its use as a root repair material.
评估与30%钨酸钙(TCS + CaWO )结合的硅酸三钙基修复材料与Bio-C修复材料(Bio-C;安吉利斯公司)和MTA修复HP材料(MTA HP;安吉利斯公司)相比的组织反应。
将填充有其中一种材料或为空的聚乙烯管(对照组,CG)植入大鼠皮下组织7、15、30和60天(每组n = 32)。评估包膜厚度、炎症细胞数量、胶原蛋白含量、白细胞介素-6(IL-6)、骨钙素(OCN)、冯·科萨反应以及偏振光下的分析。除OCN外,数据采用重复测量的广义线性模型进行分析。OCN数据采用Kruskal-Wallis检验和Dunn事后检验,以及Friedman检验后再进行Nemenyi检验,显著性水平为5%。
在所有时间点,TCS + CaWO与Bio-C之间未观察到炎症细胞数量的显著差异,而在15、30和60天时,TCS + CaWO与MTA HP之间未检测到显著差异。在所有时期,TCS + CaWO与MTA HP之间的成纤维细胞数量未检测到显著差异,在60天时,这些组与CG之间也未显示出显著差异。在所有时期,生物陶瓷材料之间的IL-6免疫表达未检测到显著差异。从7天到60天,所有组中炎症细胞数量、IL-6免疫阳性细胞数量和包膜厚度显著减少,同时胶原蛋白显著增加。在所有材料周围均观察到OCN免疫标记细胞、冯·科萨阳性结构和无定形方解石沉积,而在CG中未见到这些结构。
这些发现表明,实验材料(TCS + CaWO )具有生物相容性且具有生物活性潜力,与MTA HP和Bio-C修复材料相似,并建议将其用作根管修复材料。