College of Plant Protection, Southwest University, Chongqing 400715, China.
Jiaxing Academy of Agricultural Sciences, Jiangxing 314016, China.
Phytopathology. 2023 Mar;113(3):549-558. doi: 10.1094/PHYTO-03-22-0099-R. Epub 2023 Mar 14.
Rice false smut, caused by , has become one of the most devastating grain diseases of rice worldwide. Understanding the genetic diversity of is essential for efficient disease control and breeding for disease resistance. However, little is known about the genetic variation of from different rice cultivars. We investigated the genetic diversity and pathogenic variation of isolates from 10 rice cultivars in Zhejiang, China. A total of 260 polymorphic loci and 27 haplotypes were identified based on the 2,137-bp combined DNA fragments of all individuals; hap_4 was the most common haplotype, represented by 41 isolates. Phylogeny indicated that all isolates were divided into four genetic groups. Group I was the largest, with 98 isolates, distributed mainly in eight cultivar populations, whereas 90% of the isolates collected from a Changxiang cultivar were clustered in Group IV. Furthermore, the pairwise F values exhibited significant genetic differentiation in 27 of the pairwise comparisons between populations, accounting for 23.21% of the total genetic variation. The genetic composition of the isolates of the CX population was distinguishable from that of the other nine populations, and genetic recombination was found in a few isolates. Finally, 27 haplotype representative isolates showed high variation in pathogenicity, and the isolates from the genetic subpopulation I were likely to be more virulent than those from genetic subpopulations II and III. Collectively, these findings suggest that differences in rice cultivars play an important role in the genetic variation of .
稻曲病，由 引起，已成为全球范围内水稻最具破坏性的谷物病害之一。了解 的遗传多样性对于高效的疾病控制和抗病性育种至关重要。然而，对于来自不同水稻品种的 的遗传变异知之甚少。我们调查了来自中国浙江 10 个水稻品种的 分离株的遗传多样性和致病性变异。基于所有个体的 2,137-bp 组合 DNA 片段，共鉴定出 260 个多态性位点和 27 个单倍型；hap_4 是最常见的单倍型，由 41 个分离株代表。系统发育表明，所有分离株分为四个遗传群。第一组最大，有 98 个分离株，主要分布在八个品种群体中，而来自 Changxiang 品种的 90%的分离株聚集在第四组。此外，27 个种群间的成对 F 值显示出显著的遗传分化，占总遗传变异的 23.21%。CX 种群分离株的遗传组成与其他九个种群的遗传组成不同，并且在少数分离株中发现了遗传重组。最后，27 个单倍型代表分离株表现出高度的致病性变异，来自遗传亚群 I 的分离株可能比来自遗传亚群 II 和 III 的分离株更具毒力。总的来说，这些发现表明水稻品种的差异在 的遗传变异中起着重要作用。
