父子对中Y染色体短串联重复序列（Y-STR）的下一代测序及与传统毛细管电泳的比较。
Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis.
作者信息
Bredemeyer Steffi, Roewer Lutz, Willuweit Sascha
机构信息
Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité - Universitätsmedizin Berlin, Berlin, Germany.
出版信息
Forensic Sci Res. 2021 Apr 16;7(3):484-489. doi: 10.1080/20961790.2021.1898078. eCollection 2022.
To evaluate the promising advantages of massively parallel sequencing (MPS) in our casework, we analysed a total of 33 Y-chromosomal short tandem repeats (Y-STRs) with traditional capillary electrophoresis (CE) and 25 Y-STRs using the newer MPS technology. We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits. Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations. By detecting isometric sequence variants between unrelated males, we show that sequencing Y-STRs using MPS can boost discrimination power.
为了评估大规模平行测序（MPS）在我们实际工作中的潜在优势，我们使用传统毛细管电泳（CE）分析了总共33个Y染色体短串联重复序列（Y-STR），并使用更新的MPS技术分析了25个Y-STR。我们使用商用和定制试剂盒研究了这两种技术在64对父子中的检测结果。当前的MPS技术证实了CE观察到的13个突变事件，并增进了我们对STR突变复杂性质的理解。通过检测无关男性之间的等长序列变异，我们表明使用MPS对Y-STR进行测序可以提高鉴别能力。
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