文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

比色原位杂交、组织病理学和免疫组织化学在人类皮肤样本中诊断新大陆皮肤利什曼病的比较

Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples.

作者信息

Ferreira Luiz Cláudio, Quintella Leonardo Pereira, Schubach Armando de Oliveira, Miranda Luciana de Freitas Campos, Madeira Maria de Fátima, Pimentel Maria Inês Fernandes, Vasconcellos Érica de Camargo Ferreira E, Lyra Marcelo Rosandiski, Oliveira Raquel de Vasconcellos Carvalhaes de, Menezes Rodrigo Caldas

机构信息

Anatomic Pathology Service, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil.

Laboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, Brazil.

出版信息

Trop Med Infect Dis. 2022 Nov 1;7(11):344. doi: 10.3390/tropicalmed7110344.

DOI:10.3390/tropicalmed7110344
PMID:36355886
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9695648/
Abstract

New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by . These specimens were analyzed by CISH, using a generic probe for , IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by (.) .

摘要

新大陆皮肤利什曼病（NWCL）是一种人兽共患病，由原生动物的不同物种引起。显色原位杂交（CISH）已被证明能令人满意地检测动物组织中的无鞭毛体形式的 spp.，但尚未对其用于诊断人类NWCL进行测试。本研究的目的是比较CISH、组织病理学（HP）和免疫组织化学（IHC）技术在诊断人类皮肤病变中的NWCL的效果。样本包括五十份来自因 引起的NWCL患者的福尔马林固定、石蜡包埋的皮肤活检标本。这些标本通过CISH进行分析，使用一种通用探针检测 ，通过免疫组织化学（IHC）和组织病理学（HP），以寄生虫学培养作为标准参考来评估这些方法的敏感性。还纳入了三名被诊断为皮肤真菌病患者的额外标本，以评估CISH和IHC之间的交叉反应。免疫组织化学（IHC）、显色原位杂交（CISH）和组织病理学（HP）检测无鞭毛体的敏感性分别为66%、54%和50%。与CISH不同，免疫组织化学（IHC）与不同种类的真菌发生交叉反应。总之，这些结果表明，在由 （.）引起的人类NWCL的实验室诊断常规中，显色原位杂交（CISH）可能是检测无鞭毛体的一种补充检测方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3380/9695648/771775a2f563/tropicalmed-07-00344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3380/9695648/ba3dff89aa74/tropicalmed-07-00344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3380/9695648/771775a2f563/tropicalmed-07-00344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3380/9695648/ba3dff89aa74/tropicalmed-07-00344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3380/9695648/771775a2f563/tropicalmed-07-00344-g002.jpg

相似文献

[1]
Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples.

Trop Med Infect Dis. 2022-11-1

[2]
Comparative Study of Colorimetric In Situ Hybridization and Quantitative Real-Time Polymerase Chain Reaction for Diagnosis of Infection by in Dogs in Formalin-Fixed and Paraffin-Embedded Skin.

Trop Med Infect Dis. 2024-4-22

[3]
Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis-driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method.

Trans R Soc Trop Med Hyg. 2015-7

[4]
Sensitivity and specificity of in situ hybridization for diagnosis of cutaneous infection by Leishmania infantum in dogs.

J Clin Microbiol. 2012-11-7

[5]
Performance of immunohistochemistry as a useful tool for the diagnosis of cutaneous leishmaniasis in Panama, Central America.

Parasitol Int. 2019-8

[6]
Anti-mitochondrial Tryparedoxin Peroxidase Monoclonal Antibody-Based Immunohistochemistry for Diagnosis of Cutaneous Leishmaniasis.

Front Microbiol. 2022-2-28

[7]
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.

Parasitol Int. 2017-2

[8]
Detection of Leishmania braziliensis in human paraffin-embedded tissues from Tucumán, Argentina by polymerase chain reaction.

Mem Inst Oswaldo Cruz. 2005-4

[9]
Leishmania (V.) braziliensis: detection by PCR in biopsies from patients with cutaneous leishmaniasis.

Exp Parasitol. 2008-7

[10]
Comparative studies of the detection rates of Leishmania parasites from formalin, ethanol-fixed, frozen human skin specimens by polymerase chain reaction and Southern blotting.

J Dermatol. 1998-10

引用本文的文献

[1]
Extracellular Vesicle Release from Immune Cells in Cutaneous Leishmaniasis: Modulation by and Reversal by Antimonial Therapy.

Pathogens. 2025-8-4

[2]
Performance of Culture Using a Semi-Automatic Needle as a Novel Tool for Collecting Lymph Node Samples for the Diagnosis of Canine Visceral Leishmaniasis.

Animals (Basel). 2025-1-5

[3]
Comparative Study of Colorimetric In Situ Hybridization and Quantitative Real-Time Polymerase Chain Reaction for Diagnosis of Infection by in Dogs in Formalin-Fixed and Paraffin-Embedded Skin.

Trop Med Infect Dis. 2024-4-22

[4]
The Potential Use of Peptides in the Fight against Chagas Disease and Leishmaniasis.

Pharmaceutics. 2024-2-4

[5]
Advancement in Leishmaniasis Diagnosis and Therapeutics.

Trop Med Infect Dis. 2023-5-10

本文引用的文献

[1]
Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum.

PLoS One. 2021

[2]
Diagnosing human cutaneous leishmaniasis using fluorescence hybridization.

Pathog Glob Health. 2021-7

[3]
Accuracy of diagnostic tests for American tegumentary leishmaniasis: a systematic literature review with meta-analyses.

Trop Med Int Health. 2020-10

[4]
Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis.

An Bras Dermatol. 2020

[5]
The accuracy of the Montenegro skin test for leishmaniasis in PCR-negative patients.

Rev Soc Bras Med Trop. 2020-4-27

[6]
Leishmaniasis.

Lancet. 2018-8-17

[7]
DNA degrades during storage in formalin-fixed and paraffin-embedded tissue blocks.

Virchows Arch. 2017-10

[8]
Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review.

Crit Rev Microbiol. 2017-1-27

[9]
Effectiveness of an immunohistochemical protocol for Leishmania detection in different clinical forms of American tegumentary leishmaniasis.

Parasitol Int. 2017-2

[10]
Occurrence of Leishmania infantum and associated histological alterations in the genital tract and mammary glands of naturally infected dogs.

Parasitol Res. 2016-6

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索