Brindha Kothaimanimaran, Mohanraj Sundaresan, Rajaguru Palanichamy, Pugalenthi Velan
Department of Biotechnology, University College of Engineering, Bharathidasan Institute of Technology Campus, Anna University, Tiruchirappalli 620 024, Tamil Nadu, India.
Department of Biochemistry, KMCH research foundation, Coimbatore 641014, Tamil Nadu, India.
Sci Total Environ. 2023 Feb 10;859(Pt 1):160002. doi: 10.1016/j.scitotenv.2022.160002. Epub 2022 Nov 8.
Considering the environmental impacts, rapid fossil fuel depletion and production costs, sustainable production of clean biofuels from alternative sources is required to meet the increasing demand for energy while avoiding environmental pollution. In this study, phytogenic cobalt nanoparticles (CoNPs)-assisted dark fermentation process was developed for the simultaneous production of biohydrogen, biobutanol and biopolymer from glucose using Clostridium acetobutylicum NCIM 2337. The maximum biohydrogen yield of 2.89 mol H/mol glucose was achieved at 1.5 mg of CoNPs, which is 1.6 folds higher than that of the control experiment. The high level of soluble metabolites, specifically acetate and butyrate, confirmed the production of biohydrogen through acetate/butyrate pathways. The modified Gompertz model fitted well with experimental results of CoNPs-assisted biohydrogen production. The CoNPs could act as an electron carrier in intracellular metabolism to enhance the activity of ferredoxin and hydrogenase enzymes, thus improving biohydrogen production. Furthermore, biobutanol and biopolymer yields of 975 ± 2.5 mg/L and 1182 ± 1.4 mg/L were achieved, with 2.0 mg and 2.5 mg of CoNP, respectively, which were 1.27 and 1.19 folds higher than the control values. Hence, the inclusion of CoNPs in the fermentation medium seems to be a promising technique for the enhanced simultaneous production of biohydrogen, biobutanol and biopolymer. The environmental perspectives of the obtained renewable biohydrogen, biobutanol and biopolymer are also discussed.
考虑到环境影响、化石燃料的迅速枯竭以及生产成本，需要从替代来源可持续地生产清洁生物燃料，以满足不断增长的能源需求，同时避免环境污染。在本研究中，开发了植物源钴纳米颗粒（CoNPs）辅助的暗发酵工艺，用于利用丙酮丁醇梭菌NCIM 2337从葡萄糖中同时生产生物氢、生物丁醇和生物聚合物。在1.5毫克CoNPs的情况下，生物氢的最大产量达到2.89摩尔H/摩尔葡萄糖，这比对照实验高出1.6倍。高水平的可溶性代谢产物，特别是乙酸盐和丁酸盐，证实了通过乙酸盐/丁酸盐途径生产生物氢。修正的Gompertz模型与CoNPs辅助生物制氢的实验结果拟合良好。CoNPs可以作为细胞内代谢中的电子载体，增强铁氧化还原蛋白和氢化酶的活性，从而提高生物氢的产量。此外，分别使用2.0毫克和2.5毫克CoNP时，生物丁醇和生物聚合物的产量分别达到975±2.5毫克/升和1182±1.4毫克/升，分别比对照值高出1.27倍和1.19倍。因此，在发酵培养基中加入CoNPs似乎是一种有前景的技术，可用于增强生物氢、生物丁醇和生物聚合物的同时生产。还讨论了所获得的可再生生物氢、生物丁醇和生物聚合物的环境前景。
