Choi Soyeon, Lee Hojun, Lee Min-Soo, Park Joon Tae, Heynderickx Philippe M, Wu Di, Depuydt Stephen, Asselman Jana, Janssen Colin, Häder Donat P, Han Taejun, Park Jihae
Department of Marine Science, Incheon National University, 119 Academy-ro, Incheon 22012, Korea.
Bio Environmental Science and Technology (BEST) Laboratory, Ghent University Global Campus, 119-5 Songdomunhwa-ro, Incheon 21985, Korea.
Biology (Basel). 2022 Nov 5;11(11):1618. doi: 10.3390/biology11111618.
A chemical analysis of water quality cannot detect some toxicants due to time constraints, high costs, and limited interactions for detection. Bioassays would offer a complementary means to assess pollution levels in water. is a flagellate green alga and an excellent system for toxicity testing thanks to its ease of culture, rapid growth, and quick response to environmental stresses. Herein, we examined the sensitivity of to seven heavy metals by analyzing six end-point parameters: motility, velocity, cell compactness, upward swimming, -value, and alignment. Notably, the velocity of was most sensitive to cadmium (96.28 mg·L), copper (6.51 mg·L), manganese (103.28 mg·L), lead (78.04 mg·L), and zinc (101.90 mg·L), while -values were most sensitive to arsenic (12.84 mg·L) and mercury (4.26 mg·L). In this study, velocity and -values are presented as useful biomarkers for the assessment of metal toxicity in . The metals As, Cd, Cu, and Pb were suitable for this test. The advantages of the ecotoxicity test are its rapidity: It takes 10 min to obtain results, as opposed to the typical 3-4 d of exposure time with intensive labor. Moreover, this test can be performed at room temperature under dark conditions.
由于时间限制、成本高昂以及检测时相互作用有限，水质的化学分析无法检测出某些有毒物质。生物测定法将提供一种补充手段来评估水中的污染水平。[某种藻类名称]是一种鞭毛绿藻，因其易于培养、生长迅速以及对环境压力反应迅速，是毒性测试的优良体系。在此，我们通过分析六个终点参数：运动性、速度、细胞紧实度、向上游动、[某个参数名称]值和排列，来检测[某种藻类名称]对七种重金属的敏感性。值得注意的是，[某种藻类名称]的速度对镉（96.28毫克·升）、铜（6.51毫克·升）、锰（103.28毫克·升）、铅（78.04毫克·升）和锌（101.90毫克·升）最为敏感，而[某个参数名称]值对砷（12.84毫克·升）和汞（4.26毫克·升）最为敏感。在本研究中，速度和[某个参数名称]值被视为评估[某种藻类名称]中金属毒性的有用生物标志物。砷、镉、铜和铅这几种金属适用于此测试。生态毒性测试的优点在于其快速性：只需10分钟就能得出结果，而传统的暴露时间为3 - 4天且劳动强度大。此外，该测试可在室温黑暗条件下进行。
