Watanabe Chisato, Shibuya Hirotoshi, Ichiyama Yusuke, Okamura Eiichi, Tsukiyama-Fujii Setsuko, Tsukiyama Tomoyuki, Matsumoto Shoma, Matsushita Jun, Azami Takuya, Kubota Yoshiaki, Ohji Masahito, Sugiyama Fumihiro, Takahashi Satoru, Mizuno Seiya, Tamura Masaru, Mizutani Ken-Ichi, Ema Masatsugu
Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu 520-2192, Japan.
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Research Center, Tsukuba 305-0074, Japan.
Life (Basel). 2022 Oct 28;12(11):1730. doi: 10.3390/life12111730.
Angiogenesis is a process to generate new blood vessels from pre-existing vessels and to maintain vessels, and plays critical roles in normal development and disease. However, the molecular mechanisms underlying angiogenesis are not fully understood. This study examined the roles of during development in mice. We found that and are expressed abundantly in endothelial cells at embryonic day 8.5. The generation of knock-out (KO) mice showed that disruption of resulted in lethal . Substantial numbers of KO embryos exhibited hemorrhaging. Deletion of using Tie2-Cre transgenic mice demonstrated that in hematopoietic and endothelial lineages was responsible for the phenotype. Taken together, these findings reveal that is essential for cardiovascular and brain development in mice.
血管生成是一个从已有的血管生成新血管并维持血管的过程，在正常发育和疾病中发挥关键作用。然而，血管生成背后的分子机制尚未完全明确。本研究检测了在小鼠发育过程中的作用。我们发现，在胚胎第8.5天，和在内皮细胞中大量表达。基因敲除（KO）小鼠的产生表明，的破坏导致致死性。大量的KO胚胎出现出血。使用Tie2-Cre转基因小鼠缺失表明，造血和内皮谱系中的导致了该表型。综上所述，这些发现揭示了对小鼠心血管和脑发育至关重要。
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