Bielas Rafał, Wróbel-Marek Justyna, Kurczyńska Ewa U, Neugebauer Dorota
Department of Physical Chemistry and Technology of Polymers, Faculty of Chemistry, Silesian University of Technology, Strzody 9, 44-100 Gliwice, Poland.
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland.
Materials (Basel). 2022 Nov 2;15(21):7720. doi: 10.3390/ma15217720.
A rhodamine B (RhB)-based initiator for atom transfer radical polymerization (ATRP) was synthesized and applied for preparation of poly(2-trimethylammoniumethyl methacrylate) (PChMA), poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(2-trimethylsilyloxyethyl methacrylate) (PHEMATMS). Polymer fluorescence was confirmed by determination of quantum yield by comparative method with piroxicam as the standard exhibiting dependency of emission intensity on the polymer chain hydrophilicity and the kind of solvent. The RhB functionalized polymers were used for biological tests in plant materials except for RhB-PHEMATMS because of weak fluorescence. These two polymers slightly differed in cellular localization. RhB-PChMA was mostly observed in cell walls of root tissues and cotyledon epidermis. It was also observed in cytoplasm and cell organelles of root cap cells and rhizodermis, in contrast with cytoplasm of cotyledon epidermis. RhB-PHEMA was also present in apoplast. A strong signal in protoxylem cell walls and a weak signal in cell walls of rhizodermis and cortex were visible. Moreover, it was also present in cell walls of cotyledon epidermis. However, RhB-PHEMA was mostly observed in cytoplasm and cell organelles of all root tissues and epidermis of cotyledons. Both RhB-polymers did not cause cell death which means that they can be used in living plant material.
合成了一种基于罗丹明B（RhB）的原子转移自由基聚合（ATRP）引发剂，并将其应用于制备聚（甲基丙烯酸2-三甲基铵乙酯）（PChMA）、聚（甲基丙烯酸2-羟乙酯）（PHEMA）和聚（甲基丙烯酸2-三甲基硅氧基乙酯）（PHEMATMS）。通过以吡罗昔康为标准采用比较法测定量子产率来确认聚合物荧光，结果表明发射强度取决于聚合物链亲水性和溶剂种类。除了RhB-PHEMATMS因荧光较弱外，RhB功能化聚合物用于植物材料的生物学测试。这两种聚合物在细胞定位上略有不同。RhB-PChMA主要在根组织细胞壁和子叶表皮中观察到。在根冠细胞和根表皮的细胞质和细胞器中也观察到了它，而在子叶表皮细胞质中则未观察到。RhB-PHEMA也存在于质外体中。在原生木质部细胞壁中可见强信号，在根表皮和皮层细胞壁中可见弱信号。此外，它也存在于子叶表皮细胞壁中。然而，RhB-PHEMA主要在所有根组织的细胞质和细胞器以及子叶表皮中观察到。两种RhB聚合物均未导致细胞死亡，这意味着它们可用于活的植物材料。
