Yoda Tsuyoshi
Hachinohe Industrial Research Institute, Aomori Prefectural Industrial Technology Research Center, 1-4-43 Kita-inter-kogyodanchi, Hachinohe City 039-2245, Aomori, Japan.
The United Graduate School of Agricultural Sciences, Iwate University, 3-18-8, Ueda, Morioka City 020-8550, Iwate, Japan.
Membranes (Basel). 2022 Nov 9;12(11):1121. doi: 10.3390/membranes12111121.
Positively charged ion species and charged lipids play specific roles in biochemical processes, especially those involving cell membranes. The cell membrane and phase separation domains are attractive research targets to study signal transduction. The phase separation structure and functions of cell-sized liposomes containing charged lipids and cholesterol have been investigated earlier, and the domain structure has also been studied in a membrane model, containing the yeast sterol ergosterol. The present study investigates phase-separated domain structure alterations in membranes containing charged lipids when cholesterol is substituted with ergosterol. This study finds that ergosterol increases the homogeneity of membranes containing charged lipids. Cholesterol-containing membranes are more sensitive to a charged state, and ergosterol-containing liposomes show lower responses to charged lipids. These findings may improve our understanding of the differences in both yeast and mammalian cells, as well as the interactions of proteins with lipids during signal transduction.
带正电荷的离子种类和带电荷的脂质在生化过程中发挥着特定作用，尤其是在涉及细胞膜的过程中。细胞膜和相分离结构域是研究信号转导的有吸引力的研究目标。早期已经研究了含有带电荷脂质和胆固醇的细胞大小脂质体的相分离结构和功能，并且在含有酵母固醇麦角固醇的膜模型中也研究了结构域结构。本研究调查了当胆固醇被麦角固醇取代时，含有带电荷脂质的膜中相分离结构域结构的变化。该研究发现，麦角固醇增加了含有带电荷脂质的膜的均匀性。含胆固醇的膜对带电状态更敏感，而含麦角固醇的脂质体对带电荷脂质的反应较低。这些发现可能会增进我们对酵母细胞和哺乳动物细胞差异以及信号转导过程中蛋白质与脂质相互作用的理解。
