Department of Bioinformatics and Biotechnology, Government College University Faisalabad (GCUF), Allama Iqbal Road, Faisalabad 38000, Pakistan.
Department of Biochemistry, Government College University Faisalabad (GCUF), Jhang Road, Faisalabad 38000, Pakistan.
Molecules. 2022 Oct 25;27(21):7220. doi: 10.3390/molecules27217220.
Lactic acid bacteria produce a variety of antibacterial and larvicidal metabolites, which could be used to cure diseases caused by pathogenic bacteria and to efficiently overcome issues regarding insecticide resistance. In the current study, the antibacterial and larvicidal potential of Bis-(2-ethylhexyl) phthalate isolated from BCH-1 has been evaluated. Bioactive compounds were extracted by ethyl acetate and were fractionated by gradient column chromatography from crude extract. Based on FT-IR analysis followed by GC-MS and ESI-MS/MS, the active compound was identified to be Bis-(2-ethylhexyl) phthalate. Antibacterial potential was evaluated by disk diffusion against (12.33 ± 0.56 mm inhibition zone) and (5.66 ± 1.00 mm inhibition zone). Larvicidal potency was performed against Say larvae, where Bis-(2-ethylhexyl) phthalate showed 100% mortality at 250 ppm after 72 h with LC of 67.03 ppm. Furthermore, after 72 h the acetylcholinesterase inhibition was observed as 29.00, 40.33, 53.00, 64.00, and 75.33 (%) at 50, 100, 150, 200, and 250 ppm, respectively. In comet assay, mean comet tail length (14.18 ± 0.28 μm), tail DNA percent damage (18.23 ± 0.06%), tail movement (14.68 ± 0.56 µm), comet length (20.62 ± 0.64 µm), head length (23.75 ± 0.27 µm), and head DNA percentage (39.19 ± 0.92%) were observed at 250 ppm as compared to the control. The current study for the first time describes the promising antibacterial and larvicidal potential of Bis-(2-ethylhexyl) phthalate from that would have potential pharmaceutical applications.
从 BCH-1 中分离出的邻苯二甲酸二(2-乙基己基)酯具有抗菌和杀幼虫作用。用乙酸乙酯提取生物活性化合物,用梯度柱色谱法从粗提物中分离。根据 FT-IR 分析、GC-MS 和 ESI-MS/MS,确定活性化合物为邻苯二甲酸二(2-乙基己基)酯。抑菌活性采用抑菌圈法测定,对 和 的抑菌圈直径分别为(12.33 ± 0.56mm)和(5.66 ± 1.00mm)。杀幼虫活性对 Say 幼虫进行测定,邻苯二甲酸二(2-乙基己基)酯在 250ppm 时,72h 后死亡率为 100%,LC 为 67.03ppm。此外,在 72h 时,乙酰胆碱酯酶抑制率分别为 50、100、150、200 和 250ppm 时的 29.00%、40.33%、53.00%、64.00%和 75.33%。在彗星试验中,250ppm 时的平均彗星尾长(14.18 ± 0.28μm)、尾 DNA 损伤百分比(18.23 ± 0.06%)、尾移动距离(14.68 ± 0.56μm)、彗星长度(20.62 ± 0.64μm)、头长度(23.75 ± 0.27μm)和头 DNA 百分比(39.19 ± 0.92%)均高于对照组。本研究首次描述了 中邻苯二甲酸二(2-乙基己基)酯具有良好的抗菌和杀幼虫作用,具有潜在的药用价值。