Sharma Neha, Kar Anand, Panda Sunanda, Yadav Dhananjay
School of Life Sciences, Devi Ahilya University, Khandwa Road, Indore, 452017, India.
Department of Life Sciences, Yeungnam University, Gyeongsan, 38541, South Korea.
Curr Top Med Chem. 2022;22(32):2617-2628. doi: 10.2174/1568026623666221108125036.
Diabetes mellitus (DM) is a well-known global metabolic disorder. For its treatment, glibenclamide (GLB) is very often prescribed. However, herbal drugs are considered effective and better alternatives due to their low risk of side effects. This study was conducted to determine the combined effects of GLB and Pterocarpus marsupium (PM, a commonly available Indian herb) extract for the effective and safe treatment of hyperglycemia in the mouse model.
Healthy adult male mice were distributed into five groups (n=7 in each group). Group I acted as the control, whereas groups II, III, IV, and V were considered experimental groups which received a single dosage (150 mg/kg body weight) of alloxan (ALX) intraperitoneally (i.p.). In addition, groups III, IV, and V received a pre-standardized dose of GLB (500 μg/kg body weight), PM extract (150 mg/kg body weight), and GLB+PM, respectively, at the same doses as used in individual treatment, after the seventh day of ALX administration for 15 days and the alterations in different DM related parameters were evaluated.
ALX-induced hyperglycemia and other adverse effects were nearly normalized by GLB and PM co-treatment as evidenced by marked suppression in glucose, triglyceride, total-cholesterol, lipid-peroxidation, and lipid-hydroperoxides with an increase in antioxidants status and liver glycogen content. The positive effects were more pronounced when both GLB and PM were given, as compared to that of either of the drugs, administered alone. Liver ultra-structure, analyzed through histology and transmission electron microscopy revealed normalization of the ALX-induced damaged hepatocytes. The presence of epicatechin, the major phytoconstituent of the PM extract, as confirmed by high-performance liquid chromatography (HPLC), is responsible for its antioxidative and glucose-lowering activities.
These findings reveal that PM, along with GLB, exhibits synergistic and better effects than the individual drug in regulating hyperglycemia and associated changes in alloxan-induced mice.
糖尿病（DM）是一种广为人知的全球代谢紊乱疾病。在其治疗中，经常会开格列本脲（GLB）。然而，草药因其副作用风险低而被认为是有效且更好的替代品。本研究旨在确定GLB和印度紫檀（PM，一种常见的印度草药）提取物联合使用对小鼠模型中高血糖进行有效和安全治疗的效果。
将健康成年雄性小鼠分为五组（每组n = 7）。第一组作为对照组，而第二、三、四和五组为实验组，腹腔注射（i.p.）单次剂量（150 mg/kg体重）的四氧嘧啶（ALX）。此外，在ALX给药第7天后，第三、四和五组分别接受与单独治疗相同剂量的GLB（500 μg/kg体重）、PM提取物（150 mg/kg体重）和GLB + PM，持续15天，并评估不同糖尿病相关参数的变化。
GLB和PM联合治疗使ALX诱导的高血糖和其他不良反应几乎恢复正常，表现为葡萄糖、甘油三酯、总胆固醇、脂质过氧化和脂质氢过氧化物显著降低，抗氧化剂状态和肝糖原含量增加。与单独使用任何一种药物相比，GLB和PM联合使用时的积极效果更明显。通过组织学和透射电子显微镜分析的肝脏超微结构显示，ALX诱导的受损肝细胞恢复正常。高效液相色谱（HPLC）证实，PM提取物的主要植物成分表儿茶素具有抗氧化和降血糖活性。
这些发现表明，与GLB一起，PM在调节四氧嘧啶诱导的小鼠高血糖及相关变化方面，比单独使用药物具有协同作用且效果更好。
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