香茅醇的抗缺血作用通过促炎细胞因子介导的实验性中风模型。
Anti-ischemic Effect of Monoterpene Citronellol on Experimental Stroke Models Mediated by Pro-inflammatory Cytokines.
机构信息
Department of Neurology, The Second People`s Hospital of Liaocheng, No. 306, Jiankang Street, Linqing City, Shandong Province, 252600, China.
Department of Neurosurgery, Linqing People's Hospital, No. 317, Yaokou Street, Linqing City, Shandong Province, 252600, China.
出版信息
Comb Chem High Throughput Screen. 2023;26(10):1888-1899. doi: 10.2174/1386207326666221110092715.
BACKGROUND
Phytomedicines are proven to treat various chronic diseases as these compounds are cost-effective with few or no side effects. Elucidating the ameliorative effect of phytomedicine on cerebral ischemia may be a potent alternative therapy. Citronellol, a monoterpene alcohol, is one such phyto compound present in the essential oils of Cymbopogon nardus and Pelargonium geraniums and has immense pharmacological properties such as antihyperalgesic, anticonvulsant and antinociceptive.
OBJECTIVE
In the present work, the anti-ischemic effect of citronellol in both cellular and animal models of stroke was analyzed.
METHODS
Citronellol-pretreated SH-SY5Y cells were subjected to oxygen-glucose deprivation and reperfusion. The cells were assessed for cell viability and LDH quantification. Inflammatory cytokines were estimated in the cell lysate of citronellol pretreated OGD-R induced cells. Healthy young SD rats were pretreated with citronellol and induced with MCAO-R. The control group was comprised of sham-operated rats treated with saline. Group II was comprised of MCAO/R-induced untreated rats. Groups III and IV rats were previously treated with 10 mg/kg and 20 mg/kg citronellol, respectively, for 7 consecutive days and induced with MCAO/R. Brain edema was analyzed by quantifying the water content and the percentage of infarct was assessed using the TTC staining technique. Acetylcholinesterase activity and neurological scoring were performed to assess the neuroprotective activity of citronellol. Lipid peroxidation and antioxidant levels were quantified to evaluate the antioxidant activity of citronellol. The anti-inflammatory activity of citronellol was assessed by quantifying proinflammatory cytokines using commercially available ELISA kits.
RESULTS
Citronellol treatment significantly ameliorated neuronal damage in both cellular and animal stroke models. Prior treatment of citronellol significantly decreased the inflammatory cytokines and increased the antioxidants. Citronellol treatment effectively protected the rats from MCAO/R-induced brain edema.
CONCLUSION
Our results confirm that citronellol is an effective anti-ischemic drug with antioxidant and anti-inflammatory properties.
背景
植物药已被证明可治疗各种慢性疾病，因为这些化合物具有成本效益，且副作用很少或没有。阐明植物药对脑缺血的改善作用可能是一种有效的替代疗法。香茅醇是单萜醇的一种，存在于香茅和天竺葵的精油中，具有巨大的药理学特性，如抗痛觉过敏、抗惊厥和抗伤害感受。
目的
在本工作中，分析了香茅醇在细胞和动物中风模型中的抗缺血作用。
方法
用氧葡萄糖剥夺和再灌注处理香茅醇预处理的 SH-SY5Y 细胞。评估细胞活力和 LDH 定量。用香茅醇预处理的 OGD-R 诱导细胞的细胞裂解物中估计炎性细胞因子。用香茅醇预处理健康年轻 SD 大鼠，然后诱导 MCAO-R。对照组由用生理盐水处理的假手术大鼠组成。第 II 组由未经处理的 MCAO/R 诱导的大鼠组成。第 III 组和第 IV 组大鼠分别预先用 10mg/kg 和 20mg/kg 的香茅醇处理 7 天，然后诱导 MCAO/R。通过定量水含量分析脑水肿，并用 TTC 染色技术评估梗死百分比。通过乙酰胆碱酯酶活性和神经评分评估香茅醇的神经保护活性。通过定量使用商业可得的 ELISA 试剂盒的促炎细胞因子来评估香茅醇的抗炎活性。
结果
香茅醇治疗在细胞和动物中风模型中均显著改善神经元损伤。香茅醇的预先治疗显著降低了炎性细胞因子并增加了抗氧化剂。香茅醇治疗有效地保护大鼠免受 MCAO/R 诱导的脑水肿。
结论
我们的结果证实，香茅醇是一种有效的抗缺血药物，具有抗氧化和抗炎特性。
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