作为前列腺癌发展的可能风险因素——免疫荧光和基于分子的病例对照研究。

as a Possible Risk Factor for Prostate Cancer Development - Immunofluorescence and Molecular Based Case Control Study.

机构信息

Department of Microbiology and Immunology, Faculty of Medical Laboratory Science, 225788Omdurman Islamic University, Omdurman, Sudan.

出版信息

Cancer Control. 2022 Jan-Dec;29:10732748221140785. doi: 10.1177/10732748221140785.

DOI:10.1177/10732748221140785

PMID:36377557

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9669689/

Abstract

BACKGROUND

In vitro studies have produced conflicting results about the significance of the in the human cancers.

OBJECTIVES

Our study aims to detect the presence of Large T antigen (LTag) together with viral load quantitation in the prostate tumor samples to assess if JCV harbors risk factor for prostate cancer (PCa).

METHOD

This was a case control-based study. A total of 110 patients participated in this study, including 55 patients with PCa and another 55 patients with benign prostatic hyperplasia (BPH) as cases and controls, respectively. Tissue, blood and urine samples were collected from each participant. Tissues samples were analyzed for the presence of Ltag using a direct immunofluorescence assay (IF). Only positive IF tested samples were subjected to viral quantitation assay. Data were collected and managed using SPSS version 20.

RESULT

The LTag in the cases group was 23.63% (13/55) which was higher than that of the controls group 5.45% (3/55) with a P. value of .006 and O.R of 5.76. The mean of viral load was significantly higher among cases tissue specimens 20156 ± 5450 copies/ml compared to controls group 6378 ± 2456copies/ml with P-value of .002. The virus was detected in 11/13 (84.6%) urine samples of cases with a mean viral load of 14068 ± 4590 copies/ml compared to 2/3 (66.6%) of controls viral load 2534 ± 1267 copies/ml.

CONCLUSION

In conclusion, a higher LTag with more viral load were detected in cases group compared to controls. Our findings support a strong relationship between infection and the probability of developing PCa.

摘要

背景

体外研究对在人类癌症中的意义产生了相互矛盾的结果。

目的

我们的研究旨在检测前列腺肿瘤样本中大 T 抗原（LTag）的存在及其病毒载量定量，以评估 JCV 是否是前列腺癌（PCa）的危险因素。

方法

这是一项基于病例对照的研究。共有 110 名患者参与了这项研究，包括 55 名 PCa 患者和 55 名良性前列腺增生（BPH）患者分别作为病例和对照组。从每个参与者采集组织、血液和尿液样本。使用直接免疫荧光检测（IF）分析组织样本中 Ltag 的存在。只有 IF 检测阳性的样本才进行病毒定量检测。数据使用 SPSS 版本 20 进行收集和管理。

结果

病例组的 LTag 为 23.63%（13/55），高于对照组的 5.45%（3/55），P 值为.006，OR 为 5.76。病例组组织标本的病毒载量平均值明显高于对照组 20156±5450 拷贝/ml 比 6378±2456copies/ml，P 值为.002。在病例组中，13 例（84.6%）尿液样本中检测到病毒，病毒载量平均值为 14068±4590 拷贝/ml，而对照组中 2 例（66.6%）病毒载量为 2534±1267 拷贝/ml。