Lin Yan-Zhu, Wu You, Cao De-Hai, Peng Yong-Jian, Deng Jun, Lin Wen-Jie, Si-Tu Min-Yi, Zhuo Ling, Chen Jie-Min, Lei Man-Xia, Liu Rong-Bin, Zhang Wei-Guang, Li Jian-Jun, Yang Xiao-Chun, Feng Guo-Kai
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.
Guangdong Institute for Drug Control, Department of Biologic Products, Guangzhou, Guangdong, China.
J Clin Transl Hepatol. 2023 Feb 28;11(1):110-117. doi: 10.14218/JCTH.2021.00414. Epub 2022 Apr 12.
Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death and ranks sixth in terms of incident cases worldwide. The purpose of this study was to develop an effective and sensitive method to distinguish liver cancer tissues from normal tissues in HCC patients. Integrin α6 is a promising cell surface target for molecular imaging of HCC, where it is overexpressed and is a prognostic biomarker. We previously identified an integrin α6-targeted peptide CRWYDENAC (RWY) that has been used for positron emission tomography (PET) imaging of HCC in mouse models.
We labeled the integrin α6-targeted RWY peptide with cyanine 7 (Cy7) to form an optical probe (Cy7-RWY) for near infrared fluorescent (NIRF) and photoacoustic (PA) imaging in HCC. Mice transplanted with subcutaneous HCC-LM3 or orthotopic HCC-H22 cells that overexpressed integrin α6 were intravenously injected with Cy7-RWY and its corresponding Cy7-control. NIRF and PA images of mice were collected from 0 to 48 h after injection.
Both NIRF and PA signals started to accumulate in the tumor 2 h after injection of Cy7-RWY and peaked at 24 h.
Cy7-RWY is a promising optical probe for NIRF and PA imaging of HCC in mice, and has potential clinical application for HCC detection.
肝细胞癌（HCC）是癌症相关死亡的第四大常见原因，在全球发病病例中排名第六。本研究的目的是开发一种有效且灵敏的方法，以区分HCC患者的肝癌组织与正常组织。整合素α6是HCC分子成像中一个有前景的细胞表面靶点，它在HCC中过表达，是一种预后生物标志物。我们之前鉴定了一种整合素α6靶向肽CRWYDENAC（RWY），其已用于小鼠模型中HCC的正电子发射断层扫描（PET）成像。
我们用花菁7（Cy7）标记整合素α6靶向的RWY肽，以形成一种用于HCC近红外荧光（NIRF）和光声（PA）成像的光学探针（Cy7-RWY）。将皮下移植过表达整合素α6的HCC-LM3或原位HCC-H22细胞的小鼠静脉注射Cy7-RWY及其相应的Cy7对照。在注射后0至48小时收集小鼠的NIRF和PA图像。
注射Cy7-RWY后2小时，NIRF和PA信号开始在肿瘤中积累，并在24小时达到峰值。
Cy7-RWY是一种有前景的用于小鼠HCC的NIRF和PA成像的光学探针，对HCC检测具有潜在临床应用价值。
