利用与白蚁相关的sdu1201菌株和放线菌素D对草莓灰霉病进行生物防治。（注：原文中“with the termite associated sp. sdu1201”表述似乎不太完整规范，但大致意思如上翻译）

Biocontrol of strawberry gray mold caused by with the termite associated sp. sdu1201 and actinomycin D.

作者信息

Yong Daojing, Li Yue, Gong Kai, Yu Yingying, Zhao Shuai, Duan Qiong, Ren Cailing, Li Aiying, Fu Jun, Ni Jinfeng, Zhang Youming, Li Ruijuan

机构信息

Helmholtz International Lab for Anti-Infectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Qingdao Zhongda Agritech Co., Ltd., Qingdao, China.

出版信息

Front Microbiol. 2022 Nov 4;13:1051730. doi: 10.3389/fmicb.2022.1051730. eCollection 2022.

Strawberry gray mold caused by is one of the most severe diseases in pre- and post-harvest periods. Although fungicides have been an effective way to control this disease, they can cause serious "3R" problems (Resistance, Resurgence and Residue). In this study, sp. sdu1201 isolated from the hindgut of the fungus-growing termite revealed significant antifungal activity against . Four compounds (-) were isolated from sp. sdu1201 and further identified as actinomycins by the HRMS and 1D NMR data. Among them, actinomycin D had the strongest inhibitory activity against with the EC value of 7.65 μg mL. The control effect of actinomycin D on strawberry gray mold was also tested on fruits and leaves , and its control efficiency on leaves was 78.77% at 3 d. Moreover, actinomycin D can also inhibit the polarized growth of germ tubes of . Therefore, sp. sdu1201 and actinomycin D have great potential to gray mold as biocontrol agents.

由[具体病原菌名称未给出]引起的草莓灰霉病是采前和采后时期最严重的病害之一。尽管杀菌剂一直是控制这种病害的有效方法，但它们会引发严重的“3R”问题（抗性、再猖獗和残留）。在本研究中，从培菌白蚁后肠分离出的[具体菌种名称未给出]sp. sdu1201对[具体病原菌名称未给出]表现出显著的抗真菌活性。从[具体菌种名称未给出]sp. sdu1201中分离出四种化合物（-），并通过高分辨质谱（HRMS）和一维核磁共振（1D NMR）数据进一步鉴定为放线菌素。其中，放线菌素D对[具体病原菌名称未给出]的抑制活性最强，其半数效应浓度（EC）值为7.65 μg/mL。还在果实和叶片上测试了放线菌素D对草莓灰霉病的防治效果，在3天时其对叶片的防治效率为78.77%。此外，放线菌素D还能抑制[具体病原菌名称未给出]芽管的极性生长。因此，[具体菌种名称未给出]sp. sdu1201和放线菌素D作为生物防治剂在防治灰霉病方面具有巨大潜力。