Department of Biology, Faculty of Science, University of Sfax, Sfax, Tunisia.
Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology of Sfax, University of Sfax, Sidi Mansour Street Km 6, BP 1177, 3038, Sfax, Tunisia.
Mol Biol Rep. 2023 Feb;50(2):1157-1165. doi: 10.1007/s11033-022-08077-7. Epub 2022 Nov 22.
Prostate cancer (PCa) is the second-leading cause of mortality in men and the most commonly diagnosed non-cutaneous male malignancy. Host genetic factors, and inflammation-induced cytokines, play a key role in prostate oncogenesis. Single Nucleotide Polymorphisms (SNP) in cytokine genes were suggested to increase the susceptibility for PCa development and progression. This study aimed to investigate the association between the SNP (rs16944) in the interleukin-1 β (IL-1β) gene and the serum levels of Prostate Specific Antigen (PSA) Prolactine (PRL), testosterone, and IL-1β in Iraqi PCa patients versus healthy controls.
Taqman Real Time-PCR, was performed to investigate the IL-1β (rs16944) polymorphism in 100 Iraqi PCa patients and 50 age-matched healthy controls in a case-control study. Serum levels of PSA, PRL, and testosterone were determined by ELISA and FIA, and associated with the IL-1β serum level as well as with the SNP (rs 16944). The association between the clinico-pathological parameters and the genotype distribution of PCa patients was also studied.
There level of IL-1β was significant increased in the serum of PCa patients compared to controls (P = 8.19 10). Serum levels for other biomarkers such as PSA, PRL and testosterone were also significantly elevated in patients compared to controls (P < 0.0001). No differences were seen for genotype and allele distribution between PCa patients and controls. Nevertheless, in the group of controls, we found that 36% carried the GG genotype against only 26% in the patients group.This suggests that this could be a protective genotype (OR 0.62, P = 0.254). In addition, we found that the GA genotype is slightly more frequent in patients as compared to controls (OR 1.22, P = 0.605). Interestingly, serum levels of IL-1β, PSA, PRL and testosterone were significantly higher in PCa patients carrying the GA genotype, and the GA and AA genotypes are strongly associated with the aggressive behavior of the disease such as advanced TNM, and high Gleason score.
Our data suggest that both serum IL-1β level and IL-1β SNP (rs16944) may be considered as candidate biomarkers for PCa. Moreover, the GA, and AA genotypes carriers along with high sera levels of IL-1β, PSA and PRL, have an increased risk for PCa with aggressive behavior in Iraqi men.
前列腺癌（PCa）是男性死亡的第二大主要原因，也是男性中最常见的非皮肤恶性肿瘤。宿主遗传因素和炎症诱导的细胞因子在前列腺癌的发生中起着关键作用。细胞因子基因中的单核苷酸多态性（SNP）被认为会增加 PCa 发展和进展的易感性。本研究旨在调查白细胞介素-1β（IL-1β）基因中 SNP（rs16944）与伊拉克 PCa 患者与健康对照组的前列腺特异性抗原（PSA）催乳素（PRL）、睾酮和 IL-1β血清水平之间的关系。
采用 Taqman 实时 PCR 方法在病例对照研究中检测 100 例伊拉克 PCa 患者和 50 名年龄匹配的健康对照者的 IL-1β（rs16944）多态性。采用 ELISA 和 FIA 法测定 PSA、PRL 和睾酮的血清水平，并与 IL-1β 血清水平以及 SNP（rs16944）相关联。还研究了 PCa 患者的临床病理参数与基因型分布之间的关系。
与对照组相比，PCa 患者的血清中 IL-1β 水平显著升高（P = 8.1910）。与对照组相比，其他生物标志物如 PSA、PRL 和睾酮的血清水平也明显升高（P <0.0001）。PCa 患者和对照组之间的基因型和等位基因分布没有差异。然而，在对照组中，我们发现 36%的人携带 GG 基因型，而患者组只有 26%。这表明这可能是一种保护性基因型（OR 0.62，P = 0.254）。此外，我们发现与对照组相比，GA 基因型在患者中略为常见（OR 1.22，P = 0.605）。有趣的是，携带 GA 基因型的 PCa 患者的血清 IL-1β、PSA、PRL 和睾酮水平显著升高，GA 和 AA 基因型与疾病的侵袭性行为密切相关，如晚期 TNM 和高 Gleason 评分。
我们的数据表明，血清 IL-1β 水平和 IL-1β SNP（rs16944）均可作为 PCa 的候选生物标志物。此外，GA 和 AA 基因型携带者以及高水平的 IL-1β、PSA 和 PRL 血清与伊拉克男性具有侵袭性行为的 PCa 风险增加有关。
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