Zhang Xin, Xiao Zhuang, Zhang Jintao, Xu Changtong, Liu Siyu, Cheng Li, Zhou Shushu, Zhao Shuqin, Zhang Yuqi, Wu Jianping, Wang Ying, Liu Mingxi
State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 211166, China.
State Key Laboratory of Reproductive Medicine, Animal Core Facility of Nanjing Medical University, Nanjing 211166, China.
Cell Rep. 2022 Nov 22;41(8):111683. doi: 10.1016/j.celrep.2022.111683.
The T-shaped radial spoke (RS) is a protein complex attached to the A microtubule of the outer doublet microtubules, and it extends toward the central pair (CP). It modulates the beat frequency, amplitude, and waveform of the flagella and cilia by serving as a mechanochemical signal transducer between the CP and dyneins. In humans, RS defects cause primary ciliary dyskinesia, but the structural components of triple RSs (RS1, RS2, and RS3) in mammals remain to be elucidated in detail. Here, we introduce a mouse model that lacked the entire RS1 in sperm flagella, due to the deletion of Iqub, while the tracheal cilia possessed intact triple RSs. Furthermore, the absence of IQUB only resulted in male infertility, owing to the sperm motility defect. Based on the mouse model, the RS1 compositions are identified in sperm flagella. In summary, this study elucidates the RS1 components and function in mammalian flagella.
T形径向辐条（RS）是一种附着在外双联微管A微管上的蛋白质复合体，它向中央对（CP）延伸。它通过作为CP和动力蛋白之间的机械化学信号转导器来调节鞭毛和纤毛的搏动频率、振幅和波形。在人类中，RS缺陷会导致原发性纤毛运动障碍，但哺乳动物中三联RS（RS1、RS2和RS3）的结构成分仍有待详细阐明。在此，我们介绍一种小鼠模型，由于Iqub的缺失，精子鞭毛中缺乏完整的RS1，而气管纤毛拥有完整的三联RS。此外，由于精子运动缺陷，IQUB的缺失仅导致雄性不育。基于该小鼠模型，确定了精子鞭毛中的RS1组成。总之，本研究阐明了哺乳动物鞭毛中RS1的成分和功能。
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