Technical Innovation Center for Utilization of Marine Biological Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China.
Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China.
Mar Drugs. 2022 Nov 12;20(11):709. doi: 10.3390/md20110709.
A novel approach to producing high-purity fucoxanthinol (FXOH) was exploited as a sustainable method to maximize fucoxanthin (FX) utilization. Through fusing the genes of cholesterol esterase and SpyTag and then expressing them in , the fusion chimera was self-assembled into insoluble active aggregates by SpyTag, which could be regarded as carrier-free immobilization. The immobilization yield of the active cholesterol esterase aggregates could reach 60%. They have expressed good activity retention at 92.48% and 60.13% after 3 and 12 cycles, respectively, which is an exciting finding. The conversion ratio of FX to FXOH is 95.02%, which is remarkably higher than those realized via the conventional chemical reduction method (55.86%) and the enzymatic hydrolysis method by free cholesterol esterases (84.51%). The purity of FXOH obtained by this method is as high as 98%, which is much higher than those obtained by other methods. Thus, a promising method for simultaneously purifying and immobilizing active cholesterol esterase aggregates is demonstrated in this study by SpyTag tailoring. In addition, this study provides an eco-friendly method for producing high-purity FXOH from FX in a highly efficient manner.
本研究探索了一种新颖的方法来生产高纯度岩藻黄醇（FXOH），这是一种可持续的方法，可以最大限度地利用岩藻黄素（FX）。通过融合胆固醇酯酶和 SpyTag 的基因，并在 中表达它们，融合的嵌合体通过 SpyTag 自组装成不溶性的活性聚集体，可以被视为无载体的固定化。活性胆固醇酯酶聚集体的固定化产率可达到 60%。它们在 3 次和 12 次循环后分别保持了 92.48%和 60.13%的良好活性保留率，这是一个令人兴奋的发现。FX 到 FXOH 的转化率为 95.02%，显著高于传统的化学还原法（55.86%）和游离胆固醇酯酶的酶解方法（84.51%）。通过这种方法获得的 FXOH 的纯度高达 98%，远高于其他方法。因此，本研究通过 SpyTag 修饰，展示了一种同时纯化和固定化活性胆固醇酯酶聚集体的有前途的方法。此外，本研究还提供了一种从 FX 高效生产高纯度 FXOH 的环保方法。
Zotero 插件
