Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
Tianjin Agricultural University, Tianjin 300384, PR China; State Key Laboratory of Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, PR China.
Food Chem. 2023 Apr 16;406:134976. doi: 10.1016/j.foodchem.2022.134976. Epub 2022 Nov 17.
Laba garlic is a kind of garlic (Allium sativum L.) product and blue pigment fraction (BPF) is the characteristic fraction of Laba garlic. The objective of the study was to isolate BPF from Laba garlic and explore its stability, composition, antioxidant activity, and immunomodulatory activity. The results suggested BPF was unstable under alkaline conditions. Twenty-four constituents including 9 peptides and 10 saponins were detected in BPF by Q Exactive HF LC/MS anlaysis. BPF showed antioxidant activity in a dose-dependent manner. It also showed effective immunomodulatory activity at a concentration of 5 μg/mL at the cellular level and the morphology of RAW 264.7 cells changed to a polygonal and dendritic-like structure. BPF could significantly increase NO production (P < 0.05), and up-regulate the mRNA levels of TNF-α, IL-6, iNOS and NF-κB in the RT-QPCR analysis. The present study systematically analyzed the compositions of BPF for the first time, and the results suggested that BPF might be a potential immunomodulator candidate, which is beneficial for the development and application of garlic products and natural pigments.
腊八蒜是大蒜（Allium sativum L.）制品的一种，而蓝色素部位（BPF）是腊八蒜的特征部位。本研究旨在从腊八蒜中分离 BPF，并探究其稳定性、组成、抗氧化活性和免疫调节活性。结果表明，BPF 在碱性条件下不稳定。通过 Q Exactive HF LC/MS 分析，在 BPF 中检测到包括 9 种肽和 10 种皂苷在内的 24 种成分。BPF 具有剂量依赖性抗氧化活性。在细胞水平上，BPF 在 5μg/mL 浓度下表现出有效的免疫调节活性，RAW 264.7 细胞的形态变为多边形和树突状结构。BPF 可显著增加 NO 的产生（P<0.05），并在 RT-QPCR 分析中上调 TNF-α、IL-6、iNOS 和 NF-κB 的 mRNA 水平。本研究首次系统地分析了 BPF 的组成，结果表明 BPF 可能是一种潜在的免疫调节剂候选物，这有利于大蒜制品和天然色素的开发和应用。
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