Department of Biotechnology, Institute of Science, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh, 530 045, India.
Department of Biotechnology, Institute of Science, GITAM (Deemed to be University), Visakhapatnam, Andhra Pradesh, 530 045, India.
J Therm Biol. 2022 Dec;110:103353. doi: 10.1016/j.jtherbio.2022.103353. Epub 2022 Oct 3.
Silkworms have limited ability to regulate their body temperature upon exposure to varying atmospheric temperatures. Environmental changes, such as global warming, adversely affect silkworm viability. Spermidine, a polyamine, protects various organisms against heat stress. This study aimed to evaluate the effect of spermidine on the thermotolerance of Bombyx mori larvae. 5th instar Bombyx mori larvae were divided into the control and spermidine groups and reared at 28 ± 1 °C and 80%-85% relative humidity. To induce heat stress, the larvae were exposed to various temperatures 32 °C, 36 °C, and 40 °C for 1 h on day 5 and subsequently allowed to recover at 28 ± 1 °C. Growth characteristics were evaluated by examining larval viability and quantifying proteins and carbohydrates. The thermotolerance of the spermidine group was higher than that of the control group at 40 °C heat stress conditions. The spermidine feeding increased the protein content and reduced the carbohydrate content significantly under heat stress condition. For the first time, this study demonstrated that spermidine alleviated thermal stress by enhancing the nutritional indices and antioxidant potential of the Bombyx mori larvae. A significant increase in economic properties was observed in spermidine fed groups. Thus, foliar feeding of spermidine to B. mori larvae markedly improved silkworm thermotolerance.
家蚕在暴露于不同大气温度时，调节体温的能力有限。环境变化，如全球变暖，会对家蚕的生存能力产生不利影响。多胺精胺可以保护各种生物体免受热应激的影响。本研究旨在评估精胺对家蚕幼虫耐热性的影响。将 5 龄家蚕幼虫分为对照组和精胺组，在 28±1°C 和 80%-85%相对湿度下饲养。为了诱导热应激，将幼虫在第 5 天暴露于 32°C、36°C 和 40°C 等不同温度下 1 小时，然后在 28±1°C 下恢复。通过检查幼虫的存活率和定量蛋白质和碳水化合物来评估生长特性。在 40°C 热应激条件下，精胺组的耐热性高于对照组。在热应激条件下，精胺喂养显著增加了蛋白质含量，降低了碳水化合物含量。本研究首次表明，精胺通过提高家蚕幼虫的营养指数和抗氧化潜力来缓解热应激。在精胺喂养组中观察到经济特性的显著增加。因此，向家蚕幼虫叶面喷施精胺可显著提高家蚕的耐热性。
