CRISPR-Cas和Argonaute的流产感染功能。

The abortive infection functions of CRISPR-Cas and Argonaute.

作者信息

Chen Yu, Zeng Zhifeng, She Qunxin, Han Wenyuan

机构信息

State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China.

CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Jimo, 266237, Qingdao, China.

出版信息

Trends Microbiol. 2023 Apr;31(4):405-418. doi: 10.1016/j.tim.2022.11.005. Epub 2022 Nov 30.

DOI:10.1016/j.tim.2022.11.005

PMID:36463018

Abstract

CRISPR-Cas and prokaryotic Argonaute (pAgo) are nucleic acid (NA)-guided defense systems that protect prokaryotes against the invasion of mobile genetic elements. Previous studies established that they are directed by NA fragments (guides) to recognize invading complementary NA (targets), and that they cleave the targets to silence the invaders. Nevertheless, growing evidence indicates that many CRISPR-Cas and pAgo systems exploit the abortive infection (Abi) strategy to confer immunity. The CRISPR-Cas and pAgo Abi systems typically sense invaders using the NA recognition ability and activate various toxic effectors to kill the infected cells to prevent the invaders from spreading. This review summarizes the diverse mechanisms of these CRISPR-Cas and pAgo systems, and highlights their critical roles in the arms race between microbes and invaders.

摘要

CRISPR-Cas和原核生物Argonaute（pAgo）是核酸（NA）引导的防御系统，可保护原核生物免受移动遗传元件的入侵。先前的研究表明，它们由NA片段（向导）引导以识别入侵的互补NA（靶标），并且它们切割靶标以使入侵者沉默。然而，越来越多的证据表明，许多CRISPR-Cas和pAgo系统利用流产感染（Abi）策略来赋予免疫能力。CRISPR-Cas和pAgo Abi系统通常利用NA识别能力来感知入侵者，并激活各种毒性效应物以杀死受感染的细胞，从而防止入侵者扩散。这篇综述总结了这些CRISPR-Cas和pAgo系统的多种机制，并强调了它们在微生物与入侵者之间的军备竞赛中的关键作用。