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通过介质阻挡放电(DBD)等离子体使大豆分离蛋白与羧甲基纤维素共轭。

Conjugation of soy protein isolate with carboxymethyl cellulose through dielectric barrier discharge (DBD) plasma.

作者信息

Sharafodin Hedieh, Soltanizadeh Nafiseh, Barahimi Mohammad Sadegh

机构信息

Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.

Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.

出版信息

Food Chem. 2023 May 1;407:135059. doi: 10.1016/j.foodchem.2022.135059. Epub 2022 Nov 24.

Abstract

The present study aimed to evaluate the physicochemical properties of dielectric barrier discharge (DBD) plasma grafted carboxymethyl cellulose (CMC) and soy protein isolate (SPI). Therefore, the mixture of SPI and CMC was treated at 16, 18 and 20 kV for 5, 10 and 15 min with DBD plasma. The results of FTIR, XRD, FESEM and SDS-PAGE confirmed the SPI-CMC conjugate formation after plasma treatment, and a glycation degree of about 21 % was obtained after 15 min treatment at 18 kV. Significantly higher levels of emulsifying activity and stability, as well as solubility, were obtained for the conjugates, as compared with the SPI-CMC mixture. Also, the smaller droplet sizes were observed in emulsions obtained from conjugate produced at 18 kV for 5 min, which had the most stability after 14 days of storage at 4 °C. Eventually, it was detected that DBD plasma could graft SPI and CMC in a short time.

摘要

本研究旨在评估介质阻挡放电(DBD)等离子体接枝羧甲基纤维素(CMC)和大豆分离蛋白(SPI)的物理化学性质。因此,采用DBD等离子体在16、18和20 kV下对SPI和CMC的混合物分别处理5、10和15分钟。傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)、场发射扫描电子显微镜(FESEM)和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)的结果证实了等离子体处理后SPI-CMC共轭物的形成,在18 kV下处理15分钟后获得了约21%的糖化度。与SPI-CMC混合物相比,共轭物的乳化活性、稳定性以及溶解度均显著提高。此外,在18 kV下处理5分钟得到的共轭物所形成的乳液中观察到较小的液滴尺寸,该乳液在4℃储存14天后具有最高的稳定性。最终,检测发现DBD等离子体能够在短时间内接枝SPI和CMC。

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