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对一种编码多重耐药性的整合性接合元件的水平基因转移动力学的研究。 (注:原文结尾不完整,句子未说完,仅根据现有内容翻译)

Examination of the horizontal gene transfer dynamics of an integrative and conjugative element encoding multidrug resistance in .

作者信息

Farghaly Mai, Hynes Michael F, Nazari Mohammad, Checkley Sylvia, Liljebjelke Karen

机构信息

Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.

Department of Biological Sciences, Faculty of Science, University of Calgary, Calgary, AB, Canada.

出版信息

Can J Microbiol. 2023 Mar 1;69(3):123-135. doi: 10.1139/cjm-2021-0349. Epub 2022 Dec 10.

Abstract

Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that play a significant role in disseminating antimicrobial resistance between bacteria via horizontal gene transfer. A recently identified ICE in a clinical isolate of () is 72 914 base pairs in length and harbours seven predicted antimicrobial resistance genes conferring resistance to tetracycline (), florfenicol (), sulfonamide (), aminoglycosides (), and copper (). This study investigated  host range, assessed effects of antimicrobial stressors on transfer frequency, and examined effects of acquisition on hosts. Conjugation assays examined transfer frequency of  to and  strains. Polymerase chain reaction assays confirmed the presence of a circular intermediate, ICE-associated core genes, and cargo genes in recipient strains. Susceptibility testing examined -associated resistance phenotypes in recipient strains. Tetracycline and ciprofloxacin induction significantly increased the transfer rates of  in vitro. The copy numbers of the circular intermediate of per chromosome exhibited significant increases of ∼37-fold after tetracycline exposure and ∼4-fold after ciprofloxacin treatment. The acquisition of reduced the relative fitness of transconjugants (TG) by 28% ( = 0.72 ± 0.04) and the relative fitness of TG was decreased by 15% ( = 0.85 ± 0.01).

摘要

整合与接合元件(ICEs)是可自我转移的移动遗传元件,在通过水平基因转移在细菌间传播抗菌药物耐药性方面发挥着重要作用。最近在一株临床分离株中鉴定出的一种ICE长度为72914个碱基对,含有7个预测的抗菌药物耐药基因,赋予对四环素、氟苯尼考、磺胺类药物、氨基糖苷类药物和铜的耐药性。本研究调查了宿主范围,评估了抗菌应激源对转移频率的影响,并研究了ICE获得对宿主的影响。接合试验检测了该ICE向大肠埃希菌和肺炎克雷伯菌菌株的转移频率。聚合酶链反应试验证实受体菌株中存在环状中间体、ICE相关核心基因和货物基因。药敏试验检测了受体菌株中与ICE相关的耐药表型。四环素和环丙沙星诱导显著提高了该ICE在体外的转移率。四环素暴露后,每条染色体上该ICE环状中间体的拷贝数显著增加约37倍,环丙沙星处理后增加约4倍。ICE的获得使转接合子(TG)的相对适合度降低了28%(P = 0.72 ± 0.04),而肺炎克雷伯菌TG的相对适合度降低了15%(P = 0.85 ± 0.01)。

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