Zhu Zhuo, Zhang Liyuan, Li Xiyao, Zhang Qiong, Wang Shuying, Peng Yongzhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2023 Feb;369:128453. doi: 10.1016/j.biortech.2022.128453. Epub 2022 Dec 9.
Efficient nitrogen removal from municipal wastewater applying a pure biofilm system has promise. In this study, a partial nitrification anammox (PNA) pure biofilm system was established in a sequencing batch reactor with anaerobic/oxic/anoxic operation; using this reactor, robust nitrogen removal from municipal wastewater at ambient temperature was achieved with a nitrogen removal efficiency (NRE) of 93.3 %. Partial nitrification with anammox could be coupled at dominant nitrite-oxidizing bacteria (NOB) abundance by controlling ultra-low dissolved oxygen (<0.1 mg/L) in the aerobic section where the contribution to nitrogen removal was 79.4 %. Microorganisms with different oxygen affinity spatially distributed on the carrier. Ammonia-oxidizing bacteria (AOB) dominated on the surface of the carrier, while anammox bacteria dominated on the interior of the carrier, with their relative abundance increasing from 0.26 % to 1.78 %. The intercalary NOB were inhibited by the restricted oxygen transfer. Overall, this study provides a new approach to realize PNA in biofilm system.
应用纯生物膜系统从城市废水中高效去除氮具有前景。在本研究中，在一个采用厌氧/好氧/缺氧运行的序批式反应器中建立了一个部分硝化厌氧氨氧化（PNA）纯生物膜系统；利用该反应器，在环境温度下实现了从城市废水中高效去除氮，脱氮效率（NRE）达到93.3%。通过在好氧段控制超低溶解氧（<0.1mg/L），可以在优势亚硝酸盐氧化细菌（NOB）丰度下耦合部分硝化与厌氧氨氧化，好氧段对脱氮的贡献为79.4%。具有不同氧亲和力的微生物在载体上呈空间分布。氨氧化细菌（AOB）在载体表面占主导，而厌氧氨氧化细菌在载体内部占主导，其相对丰度从0.26%增加到1.78%。中间的NOB受到受限氧传递的抑制。总体而言，本研究为在生物膜系统中实现PNA提供了一种新方法。
