Robust nitrogen removal from municipal wastewater by partial nitrification anammox at ultra-low dissolved oxygen in a pure biofilm system.

Efficient nitrogen removal from municipal wastewater applying a pure biofilm system has promise. In this study, a partial nitrification anammox (PNA) pure biofilm system was established in a sequencing batch reactor with anaerobic/oxic/anoxic operation; using this reactor, robust nitrogen removal from municipal wastewater at ambient temperature was achieved with a nitrogen removal efficiency (NRE) of 93.3 %. Partial nitrification with anammox could be coupled at dominant nitrite-oxidizing bacteria (NOB) abundance by controlling ultra-low dissolved oxygen (<0.1 mg/L) in the aerobic section where the contribution to nitrogen removal was 79.4 %. Microorganisms with different oxygen affinity spatially distributed on the carrier. Ammonia-oxidizing bacteria (AOB) dominated on the surface of the carrier, while anammox bacteria dominated on the interior of the carrier, with their relative abundance increasing from 0.26 % to 1.78 %. The intercalary NOB were inhibited by the restricted oxygen transfer. Overall, this study provides a new approach to realize PNA in biofilm system.