MRI 方法在啮齿动物胰腺中成像β细胞功能。
MRI Methods for Imaging Beta-Cell Function in the Rodent Pancreas.
机构信息
Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
出版信息
Methods Mol Biol. 2023;2592:101-111. doi: 10.1007/978-1-0716-2807-2_7.
Abstract
The role of Zn ions in proper storage of insulin in β-cell granules is well-established so when insulin is secreted from β-cells stimulated by an increase in plasma glucose, free Zn ions are also released. This local increase in Zn can be detected in the pancreas of rodents in real time by the use of a zinc-responsive MR contrast agent. This method offers the opportunity to monitor β-cell function longitudinally in live rodents. The methods used in our lab are fully described in this short report and some MR images of a rat pancreas showing clearly enhanced hot spots in the tail are presented.
摘要
Zn 离子在胰岛素在β细胞颗粒中正确储存中的作用已得到充分证实，因此当胰岛素被β细胞分泌时，在血糖升高的刺激下，游离 Zn 离子也被释放出来。这种局部 Zn 浓度的增加可以通过使用锌响应性磁共振对比剂实时检测到。这种方法为在活体啮齿动物中监测β细胞功能提供了机会。我们实验室使用的方法在本简短报告中进行了详细描述，并展示了一张显示尾部明显增强热点的大鼠胰腺的磁共振图像。
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