Institute of Food Chemistry, Department of Food Chemistry (170b), University of Hohenheim, 70593, Stuttgart, Germany.
Anal Bioanal Chem. 2023 Feb;415(5):875-885. doi: 10.1007/s00216-022-04480-y. Epub 2022 Dec 20.
Furan fatty acids (FuFAs) are valuable antioxidants that are highly relevant for the protection of polyunsaturated fatty acids (PUFAs) in biological systems and food. Despite their low contributions to the total fatty acids, their widespread occurrence has been documented in food and biological samples. Like other fatty acids, FuFAs are also stored esterified, e.g., in triacylglycerols. However, FuFA-containing triacylglycerols had not been detected in lipidomics analyses. Here, we present a screening method that allows for the identification of traces of FuFA-containing triacylglycerols (TAGs) utilizing LC-Orbitrap-HRMS. Initially developed with the help of purposefully synthesized FuFA-containing TAGs, the screening method was successfully applied to the analysis of two fish oil samples and one mushroom extract sample. Several FuFA-containing TAGs could be identified by direct analysis using the method and database developed in this study.
呋喃脂肪酸（FuFAs）是有价值的抗氧化剂，对保护生物系统和食品中的多不饱和脂肪酸（PUFAs）非常重要。尽管它们在总脂肪酸中的贡献较低，但已在食品和生物样本中记录了它们的广泛存在。与其他脂肪酸一样，FuFAs 也以酯化的形式储存，例如在三酰基甘油中。然而，脂质组学分析中尚未检测到含 FuFA 的三酰基甘油（TAG）。在这里，我们提出了一种筛选方法，利用 LC-Orbitrap-HRMS 可以鉴定痕量含 FuFA 的三酰基甘油（TAG）。该筛选方法最初是在有目的合成的 FuFA 含量的 TAG 的帮助下开发的，该方法成功地应用于两种鱼油样品和一种蘑菇提取物样品的分析。通过使用本研究中开发的方法和数据库进行直接分析，可以鉴定出几种含 FuFA 的 TAG。
