格拉汉姆和卡诺夫斯基辣根过氧化物酶超微结构法：JHC 引文经典中的佼佼者。

The Graham and Karnovsky Horseradish Peroxidase Ultrastructural Method: A Premier JHC Citation Classic.

机构信息

Department of Cell Biology and Physiology and The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, Kansas.

出版信息

J Histochem Cytochem. 2023 Jan;71(1):43-45. doi: 10.1369/00221554221146838. Epub 2022 Dec 21.

DOI:10.1369/00221554221146838

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9912348/

Abstract

This commentary briefly reviews the background for the development of the horseradish peroxidase-diaminobenzidine tetrahydrochloride histochemical method originally described by Graham and Karnovsky in their citation classic, reprinted in full in this issue of . Some of the method's subsequent applications, including its use as a macromolecular tracer for kidney glomerular permeability and use in immunoelectron microscopy and other immunoassays, are also discussed.

摘要

本文简要回顾了辣根过氧化物酶-二氨基联苯胺四盐酸盐组织化学方法的发展背景，该方法最初由 Graham 和 Karnovsky 在他们的经典文献中描述，全文转载于本期杂志。本文还讨论了该方法的一些后续应用，包括将其作为肾脏肾小球通透性的大分子示踪剂，以及在免疫电子显微镜和其他免疫测定中的应用。

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