Li Wenwen, Qiu Zhichang, Ma Yue, Zhang Bin, Li Lingyu, Li Qiulin, He Qiuxia, Zheng Zhenjia
Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Institute of Agri-Food Processing and Nutrition, Ministry of Agriculture and Rural Affairs, Beijing 100097, China.
Antioxidants (Basel). 2022 Nov 25;11(12):2331. doi: 10.3390/antiox11122331.
The present study aimed to explore the improvement of the bioactivity of ginger peel polysaccharides (GPs) by the modification of zinc after structural characterization. The obtained GP-Zn (II) complexes consisted dominantly of glucose and galactose in a mass proportion of 95.10:2.10, with a molecular weight of 4.90 × 10 Da and a Zn content of 21.17 mg/g. The chelation of GPs and Zn (II) was mainly involved in the O-H of hydroxyl groups, and this interaction reduced the crystallinity and decreased the asymmetry of GPs, with a slight effect on the thermal stability. The administration of GPs and their Zn (II) complexes effectively alleviated CuSO-induced inflammatory response in zebrafish (: ) down-regulating the mRNA expression levels of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-12 and TNF-) and upregulating the expression of anti-inflammatory cytokine (IL-10). Furthermore, the modification of Zn (II) enhanced the inflammation-inhibiting effect of polysaccharides. Therefore, GP-Zn (II) complexes could be applied as a candidate anti-inflammatory agent for the treatment of chronic inflammation-related diseases.
本研究旨在通过锌修饰对姜皮多糖（GPs）进行结构表征后，探索其生物活性的改善情况。所获得的GP-Zn（II）络合物主要由葡萄糖和半乳糖组成，质量比为95.10:2.10，分子量为4.90×10 Da，锌含量为21.17 mg/g。GPs与Zn（II）的螯合主要涉及羟基的O-H，这种相互作用降低了GPs的结晶度，减少了其不对称性，对热稳定性影响较小。给予GPs及其Zn（II）络合物可有效减轻硫酸铜诱导的斑马鱼炎症反应（: ），下调促炎细胞因子（IL-1、IL-6、IL-8、IL-12和TNF-）的mRNA表达水平，上调抗炎细胞因子（IL-10）的表达。此外，Zn（II）修饰增强了多糖的抗炎作用。因此，GP-Zn（II）络合物可作为治疗慢性炎症相关疾病的候选抗炎剂。
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