In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from .

India and Ethiopia employ (niger plant) as a source of edible vegetable oil. Previous studies have documented the niger plant's antioxidant properties and dietary benefits. Here, extract was obtained and ten known bioactive components (-) were isolated. The antioxidant, antidiabetic, and prebiotic properties of whole extract and isolated components of niger and the plant's ability to cooperate symbiotically with probiotic strains were examined. Compound , myricetin-3--L-rhamnoside, had the highest antioxidant capacity measured in the 2,2-diphenylpicrylhydrazyl (DPPH, 4629.76 ± 6.02 µmol Trolox equivalent/g compound) and ferric-reducing antioxidant power (FRAP, 2667.62 ± 7.5 mol Trolox equivalent/g compound) assays. The lowest -amylase and glycogen phosphorylase activities and glucose diffusion were obtained with whole extracts, whereas compounds - had moderate inhibitory effects. extract also induced the highest glucose absorption by yeast cells in the presence of 5 mM of glucose. Moreover, and incubated with -sitosterol 3--D-glucoside (compound ) showed the highest prebiotic activity score. The levels of L-(+)-lactic acid isomer in the probiotic strains were the highest in presence of the whole extract and decreased progressively in the presence of flavonoid glycosides (compounds -) and -sitosterol 3--D-glucoside. The enzymatic profile of the probiotic strains was unaffected by the niger extract and compounds -. The findings revealed that the biological activities of extract are mediated by the compounds -, and it may be considered as a promising plant for the treatment of diabetes mellitus