Department of Pathophysiology, Sepsis Translational Medicine Key Laboratory of Hunan Province, National Medicine Functional Experimental Teaching Center, Xiangya School of Medicine, Central South University, Changsha 410008, China.
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
Genes (Basel). 2022 Nov 30;13(12):2258. doi: 10.3390/genes13122258.
tRNA-derived small RNAs (tsRNAs) as a novel non-coding RNA have been studied in many cardiovascular diseases, but the relationship between tsRNAs and septic cardiomyopathy has not been investigated. We sought to analyze changes of the expression profile of tsRNAs in septic cardiomyopathy and reveal an important role for tsRNAs. We constructed a sepsis model by cecal ligation and puncture (CLP) in mice, and microarray analysis was used to find differentially expressed tsRNAs. Quantitative real-time PCR was used to verify the expression of tsRNAs and the interference effect of angiogenin (ANG), a key nuclease producing tsRNAs. Bioinformatics analysis was used to predict target genes and functions. CCK-8 and LDH release assays were used to detect cell viability and cell death. A total of 158 tsRNAs were screened, of which 101 were up-regulated and 57 were down-regulated. A total of 8 tsRNAs were verified by qPCR, which was consistent with microarray results. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses suggest that these tsRNAs may be associated with the Wnt signaling pathway and participate in cellular process. The expression of tsRNAs decreased after the interference of the key nuclease ANG, while CCK-8 suggested a corresponding decrease in cell viability and an increase in the release of LDH (cell death), indicating that tsRNAs can protect cardiomyocytes during the development of septic cardiomyopathy, reduced cardiomyocyte death. : A total of 158 tsRNAs changed significantly in septic cardiomyopathy, and these tsRNAs may play a protective role in the development of septic cardiomyopathy.
tRNA 衍生的小 RNA（tsRNAs）作为一种新型非编码 RNA，已在许多心血管疾病中进行了研究，但 tsRNAs 与脓毒症性心肌病的关系尚未得到研究。我们试图分析 tsRNAs 在脓毒症性心肌病中的表达谱变化，并揭示 tsRNAs 的重要作用。我们通过盲肠结扎和穿刺（CLP）在小鼠中构建了脓毒症模型，并使用微阵列分析来寻找差异表达的 tsRNAs。实时定量 PCR 用于验证 tsRNAs 的表达和关键核酶产生 tsRNAs 的血管生成素（ANG）的干扰效应。生物信息学分析用于预测靶基因和功能。CCK-8 和 LDH 释放测定用于检测细胞活力和细胞死亡。共筛选出 158 个 tsRNAs，其中 101 个上调，57 个下调。通过 qPCR 验证了总共 8 个 tsRNAs，结果与微阵列结果一致。基因本体（GO）注释和京都基因与基因组百科全书（KEGG）途径富集分析表明，这些 tsRNAs 可能与 Wnt 信号通路有关，并参与细胞过程。关键核酶 ANG 干扰后 tsRNAs 的表达减少，而 CCK-8 提示细胞活力相应降低，LDH（细胞死亡）释放增加，表明 tsRNAs 可在脓毒症性心肌病发展过程中保护心肌细胞，减少心肌细胞死亡。总的来说，脓毒症性心肌病中有 158 个 tsRNAs 发生了显著变化，这些 tsRNAs 可能在脓毒症性心肌病的发展中发挥保护作用。
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